Second-harmonic optical diffraction tomography
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arXiv
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| Main Authors: | , , , |
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| Format: | Preprint |
| Published: |
2024
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| _version_ | 1866909207006019584 |
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| author | Saba, Amirhossein Gigli, Carlo Pu, Ye Psaltis, Demetri |
| author_facet | Saba, Amirhossein Gigli, Carlo Pu, Ye Psaltis, Demetri |
| contents | Optical diffraction tomography (ODT) has emerged as an important label-free tool in biomedicine to measure the three-dimensional (3D) structure of a biological sample. In this paper, we describe ODT using second-harmonic generation (SHG) which is a coherent nonlinear optical process with a strict symmetry selectivity and has several advantages over traditional fluorescence methods. We report the tomographic retrieval of the 3D second-order nonlinear optical susceptibility using two-dimensional holographic measurements of the SHG fields at different illumination angles and polarization states. The method is a generalization of the conventional linear ODT to the nonlinear scenario. We demonstrate the method with a numerically simulated nanoparticle distribution and an experiment with muscle tissue fibers. Our results show that SHG ODT does not only provide an effective contrast mechanism for label-free imaging but also due to the symmetry requirement enables the visualization of properties that are not otherwise accessible. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2405_11398 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Second-harmonic optical diffraction tomography Saba, Amirhossein Gigli, Carlo Pu, Ye Psaltis, Demetri Optics Optical diffraction tomography (ODT) has emerged as an important label-free tool in biomedicine to measure the three-dimensional (3D) structure of a biological sample. In this paper, we describe ODT using second-harmonic generation (SHG) which is a coherent nonlinear optical process with a strict symmetry selectivity and has several advantages over traditional fluorescence methods. We report the tomographic retrieval of the 3D second-order nonlinear optical susceptibility using two-dimensional holographic measurements of the SHG fields at different illumination angles and polarization states. The method is a generalization of the conventional linear ODT to the nonlinear scenario. We demonstrate the method with a numerically simulated nanoparticle distribution and an experiment with muscle tissue fibers. Our results show that SHG ODT does not only provide an effective contrast mechanism for label-free imaging but also due to the symmetry requirement enables the visualization of properties that are not otherwise accessible. |
| title | Second-harmonic optical diffraction tomography |
| topic | Optics |
| url | https://arxiv.org/abs/2405.11398 |