Optical signal recording of cellular activity in optogenetic stimulation of human pulp dental cells using a twin-core fiber-based Mach-Zehnder interferometer biosensor
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arXiv
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| Main Authors: | , , , , , |
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| Format: | Preprint |
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2024
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| _version_ | 1866916282988756992 |
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| author | Akbari, Faezeh Zibaii, Mohammad Ismail Nezhad, Sara Chavoshi Layeghi, Azam Dargahi, Leila Frazao, Orlando |
| author_facet | Akbari, Faezeh Zibaii, Mohammad Ismail Nezhad, Sara Chavoshi Layeghi, Azam Dargahi, Leila Frazao, Orlando |
| contents | This paper introduces an innovative two-core fiber (TCF) optic sensor employing a Mach-Zehnder interferometer (MZI) to monitor the optogenetic response of light-sensitive human dental pulp stem cells (hDPSCs). The in-fiber MZI, formed using a segment of TCF optic, detects refractive index (RI) changes in the surrounding medium. The sensor utilizes the evanescent wave of one core as the sensing arm, necessitating a thin cladding achieved through one-sided chemical etching. This design allows the sensor to detect subtle alterations in the RI of the environment by observing displacements in the interference spectrum. The optogenetic stimulation of light-sensitive cells induces variations in ion concentrations, leading to a corresponding change in refractive index. The fabricated sensor, with a peak sensitivity of 675.74 nm/RIU within the RI range of 1.39-1.43, can detect these changes. A computer simulation validated the sensitivity and optimized fabrication parameters, exhibiting satisfactory agreement with experimental results. Spectrum displacements were recorded for both light-sensitive hDPSCs and regular hDPSCs (as a control test). Results from the experiment, analyzed and compared using data analysis software, revealed that 473 nm blue light effectively stimulated light-sensitive hDPSCs. Notably, the proposed sensor, a novel structure, demonstrated its capability to detect RI changes in the cell medium during optogenetic applications. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2406_05787 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Optical signal recording of cellular activity in optogenetic stimulation of human pulp dental cells using a twin-core fiber-based Mach-Zehnder interferometer biosensor Akbari, Faezeh Zibaii, Mohammad Ismail Nezhad, Sara Chavoshi Layeghi, Azam Dargahi, Leila Frazao, Orlando Optics Neurons and Cognition This paper introduces an innovative two-core fiber (TCF) optic sensor employing a Mach-Zehnder interferometer (MZI) to monitor the optogenetic response of light-sensitive human dental pulp stem cells (hDPSCs). The in-fiber MZI, formed using a segment of TCF optic, detects refractive index (RI) changes in the surrounding medium. The sensor utilizes the evanescent wave of one core as the sensing arm, necessitating a thin cladding achieved through one-sided chemical etching. This design allows the sensor to detect subtle alterations in the RI of the environment by observing displacements in the interference spectrum. The optogenetic stimulation of light-sensitive cells induces variations in ion concentrations, leading to a corresponding change in refractive index. The fabricated sensor, with a peak sensitivity of 675.74 nm/RIU within the RI range of 1.39-1.43, can detect these changes. A computer simulation validated the sensitivity and optimized fabrication parameters, exhibiting satisfactory agreement with experimental results. Spectrum displacements were recorded for both light-sensitive hDPSCs and regular hDPSCs (as a control test). Results from the experiment, analyzed and compared using data analysis software, revealed that 473 nm blue light effectively stimulated light-sensitive hDPSCs. Notably, the proposed sensor, a novel structure, demonstrated its capability to detect RI changes in the cell medium during optogenetic applications. |
| title | Optical signal recording of cellular activity in optogenetic stimulation of human pulp dental cells using a twin-core fiber-based Mach-Zehnder interferometer biosensor |
| topic | Optics Neurons and Cognition |
| url | https://arxiv.org/abs/2406.05787 |