Enregistré dans:
| Auteurs principaux: | , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Preprint |
| Publié: |
2024
|
| Sujets: | |
| Accès en ligne: | https://arxiv.org/abs/2406.13238 |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
| _version_ | 1866916295235076096 |
|---|---|
| author | Appel, Christian Schmeltz, Margaux Rodriguez-Fernandez, Irene Anschuetz, Lukas Nielsen, Leonard C. Panepucci, Ezequiel Marijolovic, Tomislav Wakonig, Klaus Ivanovic, Aleksandra Bonnin, Anne Leonarski, Filip Wojdyla, Justyna Tomizaki, Takashi Guizar-Sicairos, Manuel Smith, Kate Beale, John H. Glettig, Wayne McAuley, Katherine Bunk, Oliver Wang, Meitian Liebi, Marianne |
| author_facet | Appel, Christian Schmeltz, Margaux Rodriguez-Fernandez, Irene Anschuetz, Lukas Nielsen, Leonard C. Panepucci, Ezequiel Marijolovic, Tomislav Wakonig, Klaus Ivanovic, Aleksandra Bonnin, Anne Leonarski, Filip Wojdyla, Justyna Tomizaki, Takashi Guizar-Sicairos, Manuel Smith, Kate Beale, John H. Glettig, Wayne McAuley, Katherine Bunk, Oliver Wang, Meitian Liebi, Marianne |
| contents | Small Angle-X-ray Scattering Tensor Tomography (SAS-TT) is a relatively new, but powerful technique for studying the multiscale architecture of hierarchical structures, which is of particular interest for life science applications. Currently, the technique is very demanding on synchrotron beamtime, which limits its applications, especially for cases requiring a statistically relevant amount of sample. This study reports the first SAS-TT measurement at a macromolecular X-ray crystallography beamline, PX-I at the SLS, with an improvement in data acquisition time from 96 h/Mvoxel in the pilot experiments to 6 h/Mvoxel, defining a new standard for fast SAS-TT and allowing the measurement of a full tomogram in 1.2 hours. Measurements were performed on the long and lenticular process of the incus bone, one of the three human auditory ossicles. The main orientation and degree of alignment of the mineralised collagen fibrils are characterised, as well as the size and shape of the mineral particles which show relevant variations in different tissue locations. The study reveals three distinct regions of high fibril alignment, most likely important pathways of sound throughout the ossicular chain, and highlights the potential of the technique to aid in future developments in middle ear reconstructive surgery. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2406_13238 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Fast Small-Angle X-ray Scattering Tensor Tomography: An Outlook into Future Applications in Life Sciences Appel, Christian Schmeltz, Margaux Rodriguez-Fernandez, Irene Anschuetz, Lukas Nielsen, Leonard C. Panepucci, Ezequiel Marijolovic, Tomislav Wakonig, Klaus Ivanovic, Aleksandra Bonnin, Anne Leonarski, Filip Wojdyla, Justyna Tomizaki, Takashi Guizar-Sicairos, Manuel Smith, Kate Beale, John H. Glettig, Wayne McAuley, Katherine Bunk, Oliver Wang, Meitian Liebi, Marianne Medical Physics Materials Science Small Angle-X-ray Scattering Tensor Tomography (SAS-TT) is a relatively new, but powerful technique for studying the multiscale architecture of hierarchical structures, which is of particular interest for life science applications. Currently, the technique is very demanding on synchrotron beamtime, which limits its applications, especially for cases requiring a statistically relevant amount of sample. This study reports the first SAS-TT measurement at a macromolecular X-ray crystallography beamline, PX-I at the SLS, with an improvement in data acquisition time from 96 h/Mvoxel in the pilot experiments to 6 h/Mvoxel, defining a new standard for fast SAS-TT and allowing the measurement of a full tomogram in 1.2 hours. Measurements were performed on the long and lenticular process of the incus bone, one of the three human auditory ossicles. The main orientation and degree of alignment of the mineralised collagen fibrils are characterised, as well as the size and shape of the mineral particles which show relevant variations in different tissue locations. The study reveals three distinct regions of high fibril alignment, most likely important pathways of sound throughout the ossicular chain, and highlights the potential of the technique to aid in future developments in middle ear reconstructive surgery. |
| title | Fast Small-Angle X-ray Scattering Tensor Tomography: An Outlook into Future Applications in Life Sciences |
| topic | Medical Physics Materials Science |
| url | https://arxiv.org/abs/2406.13238 |