Mid-Infrared Hyperspectral Microscopy with Broadband 1-GHz Dual Frequency Combs
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arXiv
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| Format: | Preprint |
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2024
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| author | Chang, Peter Ishrak, Ragib Hoghooghi, Nazanin Egbert, Scott Lesko, Daniel Swartz, Stephanie Biegert, Jens Rieker, Gregory B. Reddy, Rohith Diddams, Scott A. |
| author_facet | Chang, Peter Ishrak, Ragib Hoghooghi, Nazanin Egbert, Scott Lesko, Daniel Swartz, Stephanie Biegert, Jens Rieker, Gregory B. Reddy, Rohith Diddams, Scott A. |
| contents | Mid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth and high signal-to-noise ratio (SNR). In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers 1000 $\mathrm{cm^{-1}}$ at $\mathrm{ν_c=2941 \; cm^{-1}}$ with 12.86 kHz spectra acquisition rate and 5 $\mathrm{μm}$ spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2407_01973 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Mid-Infrared Hyperspectral Microscopy with Broadband 1-GHz Dual Frequency Combs Chang, Peter Ishrak, Ragib Hoghooghi, Nazanin Egbert, Scott Lesko, Daniel Swartz, Stephanie Biegert, Jens Rieker, Gregory B. Reddy, Rohith Diddams, Scott A. Optics Biological Physics Mid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth and high signal-to-noise ratio (SNR). In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers 1000 $\mathrm{cm^{-1}}$ at $\mathrm{ν_c=2941 \; cm^{-1}}$ with 12.86 kHz spectra acquisition rate and 5 $\mathrm{μm}$ spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging. |
| title | Mid-Infrared Hyperspectral Microscopy with Broadband 1-GHz Dual Frequency Combs |
| topic | Optics Biological Physics |
| url | https://arxiv.org/abs/2407.01973 |