A Unified Intracellular pH Landscape with SITE-pHorin: a Quantum-Entanglement-Enhanced pH Probe
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| Autores principales: | , , , , , , , , |
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| Formato: | Preprint |
| Publicado: |
2024
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| _version_ | 1866911945596076032 |
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| author | Li, Shu-Ang Meng, Xiao-Yan Zhang, Su Zhang, Ying-Jie Yang, Run-Zhou Wang, Dian-Dian Yang, Yang Liu, Pei-Pei Kang, Jian-Sheng |
| author_facet | Li, Shu-Ang Meng, Xiao-Yan Zhang, Su Zhang, Ying-Jie Yang, Run-Zhou Wang, Dian-Dian Yang, Yang Liu, Pei-Pei Kang, Jian-Sheng |
| contents | An accurate map of intracellular organelle pH is crucial for comprehending cellular metabolism and organellar functions. However, a unified intracellular pH spectrum using a single probe is still lack. Here, we developed a novel quantum entanglement-enhanced pH-sensitive probe called SITE-pHorin, which featured a wide pH-sensitive range and ratiometric quantitative measurement capabilities. Subsequently, we measured the pH of various organelles and their sub-compartments, including mitochondrial sub-spaces, Golgi stacks, endoplasmic reticulum, lysosomes, peroxisomes, and endosomes in COS-7 cells. For the long-standing debate on mitochondrial compartments pH, we measured the pH of mitochondrial cristae as 6.60 \pm 0.40, the pH of mitochondrial intermembrane space as 6.95 \pm 0.30, and two populations of mitochondrial matrix pH at approximately 7.20 \pm 0.27 and 7.50 \pm 0.16, respectively. Notably, the lysosome pH exhibited a single, narrow Gaussian distribution centered at 4.79 \pm 0.17. Furthermore, quantum chemistry computations revealed that both the deprotonation of the residue Y182 and the discrete curvature of deformed benzene ring in chromophore are both necessary for the quantum entanglement mechanism of SITE-pHorin. Intriguingly, our findings reveal an accurate pH gradient (0.6-0.9 pH unit) between mitochondrial cristae and matrix, suggesting prior knowledge about ΔpH (0.4-0.6) and mitochondrial proton motive force (pmf) are underestimated. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2407_04232 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | A Unified Intracellular pH Landscape with SITE-pHorin: a Quantum-Entanglement-Enhanced pH Probe Li, Shu-Ang Meng, Xiao-Yan Zhang, Su Zhang, Ying-Jie Yang, Run-Zhou Wang, Dian-Dian Yang, Yang Liu, Pei-Pei Kang, Jian-Sheng Quantitative Methods Biological Physics Biomolecules Subcellular Processes An accurate map of intracellular organelle pH is crucial for comprehending cellular metabolism and organellar functions. However, a unified intracellular pH spectrum using a single probe is still lack. Here, we developed a novel quantum entanglement-enhanced pH-sensitive probe called SITE-pHorin, which featured a wide pH-sensitive range and ratiometric quantitative measurement capabilities. Subsequently, we measured the pH of various organelles and their sub-compartments, including mitochondrial sub-spaces, Golgi stacks, endoplasmic reticulum, lysosomes, peroxisomes, and endosomes in COS-7 cells. For the long-standing debate on mitochondrial compartments pH, we measured the pH of mitochondrial cristae as 6.60 \pm 0.40, the pH of mitochondrial intermembrane space as 6.95 \pm 0.30, and two populations of mitochondrial matrix pH at approximately 7.20 \pm 0.27 and 7.50 \pm 0.16, respectively. Notably, the lysosome pH exhibited a single, narrow Gaussian distribution centered at 4.79 \pm 0.17. Furthermore, quantum chemistry computations revealed that both the deprotonation of the residue Y182 and the discrete curvature of deformed benzene ring in chromophore are both necessary for the quantum entanglement mechanism of SITE-pHorin. Intriguingly, our findings reveal an accurate pH gradient (0.6-0.9 pH unit) between mitochondrial cristae and matrix, suggesting prior knowledge about ΔpH (0.4-0.6) and mitochondrial proton motive force (pmf) are underestimated. |
| title | A Unified Intracellular pH Landscape with SITE-pHorin: a Quantum-Entanglement-Enhanced pH Probe |
| topic | Quantitative Methods Biological Physics Biomolecules Subcellular Processes |
| url | https://arxiv.org/abs/2407.04232 |