High-Throughput Phenotyping using Computer Vision and Machine Learning

Fuente: arXiv
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Main Authors: Singhvi, Vivaan, Lunga, Langalibalele, Nidhi, Pragya, Keum, Chris, Prakash, Varrun
Format: Preprint
Published: 2024
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author Singhvi, Vivaan
Lunga, Langalibalele
Nidhi, Pragya
Keum, Chris
Prakash, Varrun
author_facet Singhvi, Vivaan
Lunga, Langalibalele
Nidhi, Pragya
Keum, Chris
Prakash, Varrun
contents High-throughput phenotyping refers to the non-destructive and efficient evaluation of plant phenotypes. In recent years, it has been coupled with machine learning in order to improve the process of phenotyping plants by increasing efficiency in handling large datasets and developing methods for the extraction of specific traits. Previous studies have developed methods to advance these challenges through the application of deep neural networks in tandem with automated cameras; however, the datasets being studied often excluded physical labels. In this study, we used a dataset provided by Oak Ridge National Laboratory with 1,672 images of Populus Trichocarpa with white labels displaying treatment (control or drought), block, row, position, and genotype. Optical character recognition (OCR) was used to read these labels on the plants, image segmentation techniques in conjunction with machine learning algorithms were used for morphological classifications, machine learning models were used to predict treatment based on those classifications, and analyzed encoded EXIF tags were used for the purpose of finding leaf size and correlations between phenotypes. We found that our OCR model had an accuracy of 94.31% for non-null text extractions, allowing for the information to be accurately placed in a spreadsheet. Our classification models identified leaf shape, color, and level of brown splotches with an average accuracy of 62.82%, and plant treatment with an accuracy of 60.08%. Finally, we identified a few crucial pieces of information absent from the EXIF tags that prevented the assessment of the leaf size. There was also missing information that prevented the assessment of correlations between phenotypes and conditions. However, future studies could improve upon this to allow for the assessment of these features.
format Preprint
id arxiv_https___arxiv_org_abs_2407_06354
institution arXiv
publishDate 2024
record_format arxiv
spellingShingle High-Throughput Phenotyping using Computer Vision and Machine Learning
Singhvi, Vivaan
Lunga, Langalibalele
Nidhi, Pragya
Keum, Chris
Prakash, Varrun
Computer Vision and Pattern Recognition
I.4.6; I.4.7
High-throughput phenotyping refers to the non-destructive and efficient evaluation of plant phenotypes. In recent years, it has been coupled with machine learning in order to improve the process of phenotyping plants by increasing efficiency in handling large datasets and developing methods for the extraction of specific traits. Previous studies have developed methods to advance these challenges through the application of deep neural networks in tandem with automated cameras; however, the datasets being studied often excluded physical labels. In this study, we used a dataset provided by Oak Ridge National Laboratory with 1,672 images of Populus Trichocarpa with white labels displaying treatment (control or drought), block, row, position, and genotype. Optical character recognition (OCR) was used to read these labels on the plants, image segmentation techniques in conjunction with machine learning algorithms were used for morphological classifications, machine learning models were used to predict treatment based on those classifications, and analyzed encoded EXIF tags were used for the purpose of finding leaf size and correlations between phenotypes. We found that our OCR model had an accuracy of 94.31% for non-null text extractions, allowing for the information to be accurately placed in a spreadsheet. Our classification models identified leaf shape, color, and level of brown splotches with an average accuracy of 62.82%, and plant treatment with an accuracy of 60.08%. Finally, we identified a few crucial pieces of information absent from the EXIF tags that prevented the assessment of the leaf size. There was also missing information that prevented the assessment of correlations between phenotypes and conditions. However, future studies could improve upon this to allow for the assessment of these features.
title High-Throughput Phenotyping using Computer Vision and Machine Learning
topic Computer Vision and Pattern Recognition
I.4.6; I.4.7
url https://arxiv.org/abs/2407.06354