Differential Effects of Sequence-Local versus Nonlocal Charge Patterns on Phase Separation and Conformational Dimensions of Polyampholytes as Model Intrinsically Disordered Proteins
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arXiv
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| Autores principales: | , , , |
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| Formato: | Preprint |
| Publicado: |
2024
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| _version_ | 1866910562513846272 |
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| author | Pal, Tanmoy Wessén, Jonas Das, Suman Chan, Hue Sun |
| author_facet | Pal, Tanmoy Wessén, Jonas Das, Suman Chan, Hue Sun |
| contents | Conformational properties of intrinsically disordered proteins (IDPs) are governed by a sequence-ensemble relationship. To differentiate the impact of sequence-local versus sequence-nonlocal features of an IDP's charge pattern on its conformational dimensions and its phase-separation propensity, the charge "blockiness'' $κ$ and the nonlocality-weighted sequence charge decoration (SCD) parameters are compared for their correlations with isolated-chain radii of gyration ($R_{\rm g}$s) and upper critical solution temperatures (UCSTs) of polyampholytes modeled by random phase approximation, field-theoretic simulation, and coarse-grained molecular dynamics. SCD is superior to $κ$ in predicting $R_{\rm g}$ because SCD accounts for effects of contact order, i.e., nonlocality, on dimensions of isolated chains. In contrast, $κ$ and SCD are comparably good, though nonideal, predictors of UCST because frequencies of interchain contacts in the multiple-chain condensed phase are less sensitive to sequence positions than frequencies of intrachain contacts of an isolated chain, as reflected by $κ$ correlating better with condensed-phase interaction energy than SCD. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2407_07226 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Differential Effects of Sequence-Local versus Nonlocal Charge Patterns on Phase Separation and Conformational Dimensions of Polyampholytes as Model Intrinsically Disordered Proteins Pal, Tanmoy Wessén, Jonas Das, Suman Chan, Hue Sun Biomolecules Conformational properties of intrinsically disordered proteins (IDPs) are governed by a sequence-ensemble relationship. To differentiate the impact of sequence-local versus sequence-nonlocal features of an IDP's charge pattern on its conformational dimensions and its phase-separation propensity, the charge "blockiness'' $κ$ and the nonlocality-weighted sequence charge decoration (SCD) parameters are compared for their correlations with isolated-chain radii of gyration ($R_{\rm g}$s) and upper critical solution temperatures (UCSTs) of polyampholytes modeled by random phase approximation, field-theoretic simulation, and coarse-grained molecular dynamics. SCD is superior to $κ$ in predicting $R_{\rm g}$ because SCD accounts for effects of contact order, i.e., nonlocality, on dimensions of isolated chains. In contrast, $κ$ and SCD are comparably good, though nonideal, predictors of UCST because frequencies of interchain contacts in the multiple-chain condensed phase are less sensitive to sequence positions than frequencies of intrachain contacts of an isolated chain, as reflected by $κ$ correlating better with condensed-phase interaction energy than SCD. |
| title | Differential Effects of Sequence-Local versus Nonlocal Charge Patterns on Phase Separation and Conformational Dimensions of Polyampholytes as Model Intrinsically Disordered Proteins |
| topic | Biomolecules |
| url | https://arxiv.org/abs/2407.07226 |