Observation of Aerosolization-induced Morphological Changes in Viral Capsids
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Preprint |
| Published: |
2024
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| _version_ | 1866917723409219584 |
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| author | Mall, Abhishek Munke, Anna Shen, Zhou Mazumder, Parichita Bielecki, Johan E, Juncheng Estillore, Armando Kim, Chan Letrun, Romain Lübke, Jannik Rafie-Zinedine, Safi Round, Adam Round, Ekaterina Rütten, Michael Samanta, Amit K. Sarma, Abhisakh Sato, Tokushi Schulz, Florian Seuring, Carolin Wollweber, Tamme Worbs, Lena Vagovic, Patrik Bean, Richard Mancuso, Adrian P. Loh, Ne-Te Duane Beck, Tobias Küpper, Jochen Maia, Filipe R. N. C. Chapman, Henry N. Ayyer, Kartik |
| author_facet | Mall, Abhishek Munke, Anna Shen, Zhou Mazumder, Parichita Bielecki, Johan E, Juncheng Estillore, Armando Kim, Chan Letrun, Romain Lübke, Jannik Rafie-Zinedine, Safi Round, Adam Round, Ekaterina Rütten, Michael Samanta, Amit K. Sarma, Abhisakh Sato, Tokushi Schulz, Florian Seuring, Carolin Wollweber, Tamme Worbs, Lena Vagovic, Patrik Bean, Richard Mancuso, Adrian P. Loh, Ne-Te Duane Beck, Tobias Küpper, Jochen Maia, Filipe R. N. C. Chapman, Henry N. Ayyer, Kartik |
| contents | Single-stranded RNA viruses co-assemble their capsid with the genome and variations in capsid structures can have significant functional relevance. In particular, viruses need to respond to a dehydrating environment to prevent genomic degradation and remain active upon rehydration. Theoretical work has predicted low-energy buckling transitions in icosahedral capsids which could protect the virus from further dehydration. However, there has been no direct experimental evidence, nor molecular mechanism, for such behaviour. Here we observe this transition using X-ray single particle imaging of MS2 bacteriophages after aerosolization. Using a combination of machine learning tools, we classify hundreds of thousands of single particle diffraction patterns to learn the structural landscape of the capsid morphology as a function of time spent in the aerosol phase. We found a previously unreported compact conformation as well as intermediate structures which suggest an incoherent buckling transition which does not preserve icosahedral symmetry. Finally, we propose a mechanism of this buckling, where a single 19-residue loop is destabilised, leading to the large observed morphology change. Our results provide experimental evidence for a mechanism by which viral capsids protect themselves from dehydration. In the process, these findings also demonstrate the power of single particle X-ray imaging and machine learning methods in studying biomolecular structural dynamics. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2407_11687 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Observation of Aerosolization-induced Morphological Changes in Viral Capsids Mall, Abhishek Munke, Anna Shen, Zhou Mazumder, Parichita Bielecki, Johan E, Juncheng Estillore, Armando Kim, Chan Letrun, Romain Lübke, Jannik Rafie-Zinedine, Safi Round, Adam Round, Ekaterina Rütten, Michael Samanta, Amit K. Sarma, Abhisakh Sato, Tokushi Schulz, Florian Seuring, Carolin Wollweber, Tamme Worbs, Lena Vagovic, Patrik Bean, Richard Mancuso, Adrian P. Loh, Ne-Te Duane Beck, Tobias Küpper, Jochen Maia, Filipe R. N. C. Chapman, Henry N. Ayyer, Kartik Biomolecules Image and Video Processing Single-stranded RNA viruses co-assemble their capsid with the genome and variations in capsid structures can have significant functional relevance. In particular, viruses need to respond to a dehydrating environment to prevent genomic degradation and remain active upon rehydration. Theoretical work has predicted low-energy buckling transitions in icosahedral capsids which could protect the virus from further dehydration. However, there has been no direct experimental evidence, nor molecular mechanism, for such behaviour. Here we observe this transition using X-ray single particle imaging of MS2 bacteriophages after aerosolization. Using a combination of machine learning tools, we classify hundreds of thousands of single particle diffraction patterns to learn the structural landscape of the capsid morphology as a function of time spent in the aerosol phase. We found a previously unreported compact conformation as well as intermediate structures which suggest an incoherent buckling transition which does not preserve icosahedral symmetry. Finally, we propose a mechanism of this buckling, where a single 19-residue loop is destabilised, leading to the large observed morphology change. Our results provide experimental evidence for a mechanism by which viral capsids protect themselves from dehydration. In the process, these findings also demonstrate the power of single particle X-ray imaging and machine learning methods in studying biomolecular structural dynamics. |
| title | Observation of Aerosolization-induced Morphological Changes in Viral Capsids |
| topic | Biomolecules Image and Video Processing |
| url | https://arxiv.org/abs/2407.11687 |