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| Main Authors: | , , , , |
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| Format: | Preprint |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2407.16734 |
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| _version_ | 1866929433299910656 |
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| author | Grammoustianou, Aristea Saeidi, Ali Longo, Johan Risch, Felix Ionescu, Adrian M. |
| author_facet | Grammoustianou, Aristea Saeidi, Ali Longo, Johan Risch, Felix Ionescu, Adrian M. |
| contents | Traditional detection methods of C-reactive protein (CRP) inflammation biomarker, in blood are expensive, time-consuming and labor-intensive. Such existing point-of-care CRP detection devices remain invasive, since they need blood sampling (finger-pricking or venous puncture). Here, we propose an electrochemical impedance spectroscopy (EIS)-based sensor for the real-time, fast, specific, sensitive, and label-free detection of C-reactive protein in the interstitial fluid (ISF) that can be accessed with minimally invasive microneedle arrays. The sensor has the potential to be integrated in a wearable device similar with the continuous glucose monitoring, that will detect CRP in interstitial fluid in a non-invasive, inexpensive and straightforward manner. The affinity based assay was tested in both buffer and ISF-like solution. The limit of detection achieved was 0.7 ug/mL of CRP in buffer, and 0.8 ug/mL of CRP in ISF-like solution and the sensor shows excellent linearity up to 10 ug/mL. It is worth noting that the proposed sensor operates in low sample volume (down to 5 uL), and has a response time of 100 seconds. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2407_16734 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Real time detection of C reactive protein in interstitial fluid using electrochemical impedance spectroscopy, towards wearable health monitoring Grammoustianou, Aristea Saeidi, Ali Longo, Johan Risch, Felix Ionescu, Adrian M. Medical Physics Applied Physics Traditional detection methods of C-reactive protein (CRP) inflammation biomarker, in blood are expensive, time-consuming and labor-intensive. Such existing point-of-care CRP detection devices remain invasive, since they need blood sampling (finger-pricking or venous puncture). Here, we propose an electrochemical impedance spectroscopy (EIS)-based sensor for the real-time, fast, specific, sensitive, and label-free detection of C-reactive protein in the interstitial fluid (ISF) that can be accessed with minimally invasive microneedle arrays. The sensor has the potential to be integrated in a wearable device similar with the continuous glucose monitoring, that will detect CRP in interstitial fluid in a non-invasive, inexpensive and straightforward manner. The affinity based assay was tested in both buffer and ISF-like solution. The limit of detection achieved was 0.7 ug/mL of CRP in buffer, and 0.8 ug/mL of CRP in ISF-like solution and the sensor shows excellent linearity up to 10 ug/mL. It is worth noting that the proposed sensor operates in low sample volume (down to 5 uL), and has a response time of 100 seconds. |
| title | Real time detection of C reactive protein in interstitial fluid using electrochemical impedance spectroscopy, towards wearable health monitoring |
| topic | Medical Physics Applied Physics |
| url | https://arxiv.org/abs/2407.16734 |