Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Preprint |
| Published: |
2024
|
| Subjects: | |
| Online Access: | https://arxiv.org/abs/2407.17882 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1866916336042508288 |
|---|---|
| author | Xing, Xiaodan Tang, Chunling Murdoch, Siofra Papanastasiou, Giorgos Guo, Yunzhe Xiao, Xianglu Cross-Zamirski, Jan Schönlieb, Carola-Bibiane Liang, Kristina Xiao Niu, Zhangming Fang, Evandro Fei Wang, Yinhai Yang, Guang |
| author_facet | Xing, Xiaodan Tang, Chunling Murdoch, Siofra Papanastasiou, Giorgos Guo, Yunzhe Xiao, Xianglu Cross-Zamirski, Jan Schönlieb, Carola-Bibiane Liang, Kristina Xiao Niu, Zhangming Fang, Evandro Fei Wang, Yinhai Yang, Guang |
| contents | Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell fixation, therefore it may also introduce artefects and alter endogenouous cell morphology. Some IF stains are expensive or not readily available hence hindering experiments. Recent diffusion models, which synthesise high-fidelity IF images from easy-to-acquire brightfield (BF) images, offer a promising solution but are hindered by training instability and slow inference times due to the noise diffusion process. This paper presents a novel method for the conditional synthesis of IF images directly from BF images along with cell segmentation masks. Our approach employs a Residual Diffusion process that enhances stability and significantly reduces inference time. We performed a critical evaluation against other image-to-image synthesis models, including UNets, GANs, and advanced diffusion models. Our model demonstrates significant improvements in image quality (p<0.05 in MSE, PSNR, and SSIM), inference speed (26 times faster than competing diffusion models), and accurate segmentation results for both nuclei and cell bodies (0.77 and 0.63 mean IOU for nuclei and cell true positives, respectively). This paper is a substantial advancement in the field, providing robust and efficient tools for cell image analysis. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2407_17882 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Artificial Immunofluorescence in a Flash: Rapid Synthetic Imaging from Brightfield Through Residual Diffusion Xing, Xiaodan Tang, Chunling Murdoch, Siofra Papanastasiou, Giorgos Guo, Yunzhe Xiao, Xianglu Cross-Zamirski, Jan Schönlieb, Carola-Bibiane Liang, Kristina Xiao Niu, Zhangming Fang, Evandro Fei Wang, Yinhai Yang, Guang Image and Video Processing Immunofluorescent (IF) imaging is crucial for visualizing biomarker expressions, cell morphology and assessing the effects of drug treatments on sub-cellular components. IF imaging needs extra staining process and often requiring cell fixation, therefore it may also introduce artefects and alter endogenouous cell morphology. Some IF stains are expensive or not readily available hence hindering experiments. Recent diffusion models, which synthesise high-fidelity IF images from easy-to-acquire brightfield (BF) images, offer a promising solution but are hindered by training instability and slow inference times due to the noise diffusion process. This paper presents a novel method for the conditional synthesis of IF images directly from BF images along with cell segmentation masks. Our approach employs a Residual Diffusion process that enhances stability and significantly reduces inference time. We performed a critical evaluation against other image-to-image synthesis models, including UNets, GANs, and advanced diffusion models. Our model demonstrates significant improvements in image quality (p<0.05 in MSE, PSNR, and SSIM), inference speed (26 times faster than competing diffusion models), and accurate segmentation results for both nuclei and cell bodies (0.77 and 0.63 mean IOU for nuclei and cell true positives, respectively). This paper is a substantial advancement in the field, providing robust and efficient tools for cell image analysis. |
| title | Artificial Immunofluorescence in a Flash: Rapid Synthetic Imaging from Brightfield Through Residual Diffusion |
| topic | Image and Video Processing |
| url | https://arxiv.org/abs/2407.17882 |