Dihedral Angle Adherence: Evaluating Protein Structure Predictions in the Absence of Experimental Data

Fuente: arXiv
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Main Authors: Azeem, Musa, Valafar, Homayoun
Format: Preprint
Published: 2024
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author Azeem, Musa
Valafar, Homayoun
author_facet Azeem, Musa
Valafar, Homayoun
contents Determining the 3D structures of proteins is essential in understanding their behavior in the cellular environment. Computational methods of predicting protein structures have advanced, but assessing prediction accuracy remains a challenge. The traditional method, RMSD, relies on experimentally determined structures and lacks insight into improvement areas of predictions. We propose an alternative: analyzing dihedral angles, bypassing the need for the reference structure of an evaluated protein. Our method segments proteins into amino acid subsequences and searches for matches, comparing dihedral angles across numerous proteins to compute a metric using Mahalanobis distance. Evaluated on many predictions, our approach correlates with RMSD and identifies areas for prediction enhancement. This method offers a promising route for accurate protein structure prediction assessment and improvement.
format Preprint
id arxiv_https___arxiv_org_abs_2407_18336
institution arXiv
publishDate 2024
record_format arxiv
spellingShingle Dihedral Angle Adherence: Evaluating Protein Structure Predictions in the Absence of Experimental Data
Azeem, Musa
Valafar, Homayoun
Biomolecules
Computational Engineering, Finance, and Science
Determining the 3D structures of proteins is essential in understanding their behavior in the cellular environment. Computational methods of predicting protein structures have advanced, but assessing prediction accuracy remains a challenge. The traditional method, RMSD, relies on experimentally determined structures and lacks insight into improvement areas of predictions. We propose an alternative: analyzing dihedral angles, bypassing the need for the reference structure of an evaluated protein. Our method segments proteins into amino acid subsequences and searches for matches, comparing dihedral angles across numerous proteins to compute a metric using Mahalanobis distance. Evaluated on many predictions, our approach correlates with RMSD and identifies areas for prediction enhancement. This method offers a promising route for accurate protein structure prediction assessment and improvement.
title Dihedral Angle Adherence: Evaluating Protein Structure Predictions in the Absence of Experimental Data
topic Biomolecules
Computational Engineering, Finance, and Science
url https://arxiv.org/abs/2407.18336