Propagation of Enzyme-driven Active Fluctuations in Crowded Milieu
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| Main Authors: | , , , , , , |
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| Format: | Preprint |
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2024
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| _version_ | 1866917738639785984 |
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| author | Chakraborty, Rik Maiti, Arnab Paul, Diptangshu Borthakur, Rajnandan Jayaprakash, K. R. Ghosh, Uddipta Dey, Krishna Kanti |
| author_facet | Chakraborty, Rik Maiti, Arnab Paul, Diptangshu Borthakur, Rajnandan Jayaprakash, K. R. Ghosh, Uddipta Dey, Krishna Kanti |
| contents | We investigated the energy transfer from active enzymes to their surroundings in crowded environments by measuring the diffusion of passive microscopic tracers in active solutions of ficoll and glycerol. Despite observing lower rates of substrate turnover and relatively smaller enhancement of passive tracer diffusion in artificial crowded media compared to those in aqueous solutions, we found a significantly higher relative diffusion enhancement in crowded environments in the presence of enzymatic activity. Our experimental observations, coupled with supporting analytical estimations, underscored the critical role of the intervening media in facilitating mechanical energy distribution around active enzymes. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2408_00578 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Propagation of Enzyme-driven Active Fluctuations in Crowded Milieu Chakraborty, Rik Maiti, Arnab Paul, Diptangshu Borthakur, Rajnandan Jayaprakash, K. R. Ghosh, Uddipta Dey, Krishna Kanti Soft Condensed Matter Biological Physics We investigated the energy transfer from active enzymes to their surroundings in crowded environments by measuring the diffusion of passive microscopic tracers in active solutions of ficoll and glycerol. Despite observing lower rates of substrate turnover and relatively smaller enhancement of passive tracer diffusion in artificial crowded media compared to those in aqueous solutions, we found a significantly higher relative diffusion enhancement in crowded environments in the presence of enzymatic activity. Our experimental observations, coupled with supporting analytical estimations, underscored the critical role of the intervening media in facilitating mechanical energy distribution around active enzymes. |
| title | Propagation of Enzyme-driven Active Fluctuations in Crowded Milieu |
| topic | Soft Condensed Matter Biological Physics |
| url | https://arxiv.org/abs/2408.00578 |