Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Preprint |
| Publicado: |
2024
|
| Materias: | |
| Acceso en línea: | https://arxiv.org/abs/2408.04988 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Tabla de Contenidos:
- Bacterial second messengers are crucial for transmitting environmental information to cellular responses. However, quantifying their information transmission capacity remains challenging. Here, we engineer an isolated cAMP signaling channel in Pseudomonas aeruginosa using targeted gene knockouts, optogenetics, and a fluorescent cAMP probe. This design allows precise optical control and real-time monitoring of cAMP dynamics. By integrating experimental data with information theory, we reveal an optimal frequency for light-mediated cAMP signaling that maximizes information transmission, reaching about 40 bits/h. This rate correlates strongly with cAMP degradation kinetics and employs a two-state encoding scheme. Our findings suggest a mechanism for fine-tuned regulation of multiple genes through temporal encoding of second messenger signals, providing new insights into bacterial adaptation strategies. This approach offers a framework for quantifying information processing in cellular signaling systems.