Motor-driven microtubule diffusion in a photobleached dynamical coordinate system
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arXiv
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| Autori principali: | , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| _version_ | 1866909292274122752 |
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| author | Hirokawa, Soichi Lee, Heun Jin Banks, Rachel A Duarte, Ana I Najma, Bibi Thomson, Matt Phillips, Rob |
| author_facet | Hirokawa, Soichi Lee, Heun Jin Banks, Rachel A Duarte, Ana I Najma, Bibi Thomson, Matt Phillips, Rob |
| contents | Motor-driven cytoskeletal remodeling in cellular systems can often be accompanied by a diffusive-like effect at local scales, but distinguishing the contributions of the ordering process, such as active contraction of a network, from this active diffusion is difficult to achieve. Using light-dimerizable kinesin motors to spatially control the formation and contraction of a microtubule network, we deliberately photobleach a grid pattern onto the filament network serving as a transient and dynamic coordinate system to observe the deformation and translation of the remaining fluorescent squares of microtubules. We find that the network contracts at a rate set by motor speed but is accompanied by a diffusive-like spread throughout the bulk of the contracting network with effective diffusion constant two orders of magnitude lower than that for a freely-diffusing microtubule. We further find that on micron scales, the diffusive timescale is only a factor of approximately 3 slower than that of advection regardless of conditions, showing that the global contraction and long-time relaxation from this diffusive behavior are both motor-driven but exhibit local competition within the network bulk. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2408_11216 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Motor-driven microtubule diffusion in a photobleached dynamical coordinate system Hirokawa, Soichi Lee, Heun Jin Banks, Rachel A Duarte, Ana I Najma, Bibi Thomson, Matt Phillips, Rob Biological Physics Soft Condensed Matter Motor-driven cytoskeletal remodeling in cellular systems can often be accompanied by a diffusive-like effect at local scales, but distinguishing the contributions of the ordering process, such as active contraction of a network, from this active diffusion is difficult to achieve. Using light-dimerizable kinesin motors to spatially control the formation and contraction of a microtubule network, we deliberately photobleach a grid pattern onto the filament network serving as a transient and dynamic coordinate system to observe the deformation and translation of the remaining fluorescent squares of microtubules. We find that the network contracts at a rate set by motor speed but is accompanied by a diffusive-like spread throughout the bulk of the contracting network with effective diffusion constant two orders of magnitude lower than that for a freely-diffusing microtubule. We further find that on micron scales, the diffusive timescale is only a factor of approximately 3 slower than that of advection regardless of conditions, showing that the global contraction and long-time relaxation from this diffusive behavior are both motor-driven but exhibit local competition within the network bulk. |
| title | Motor-driven microtubule diffusion in a photobleached dynamical coordinate system |
| topic | Biological Physics Soft Condensed Matter |
| url | https://arxiv.org/abs/2408.11216 |