Full-field Brillouin microscopy based on an imaging Fourier transform spectrometer
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arXiv
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| _version_ | 1866916380481159168 |
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| author | Bevilacqua, Carlo Prevedel, Robert |
| author_facet | Bevilacqua, Carlo Prevedel, Robert |
| contents | Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous Brillouin scattering results in weak signals typically requiring prolonged exposure times or illumination dosages potentially harmful for biological samples. Here, we present a new approach for highly-multiplexed, and therefore rapid, spectral acquisition of the Brillouin scattered light. Specifically, by exploiting a custom-built Fourier-transform imaging spectrometer and the symmetric properties of the Brillouin spectrum, we experimentally demonstrate full-field 2D spectral Brillouin imaging of phantoms as well as biological samples, at a throughput of up to 40,000 spectra per second over a ~300um field-of-view. This represents an approximately three orders of magnitude improvement in speed and throughput compared to standard confocal methods while retaining high spatial resolution and the capability to acquire three-dimensional images of photosensitive samples in biology and medicine. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2409_02092 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Full-field Brillouin microscopy based on an imaging Fourier transform spectrometer Bevilacqua, Carlo Prevedel, Robert Optics Brillouin microscopy is an emerging optical elastography technique that can be used to assess mechanical properties of biological samples in a 3D, all-optical and hence non-contact fashion. However, the low cross-section of spontaneous Brillouin scattering results in weak signals typically requiring prolonged exposure times or illumination dosages potentially harmful for biological samples. Here, we present a new approach for highly-multiplexed, and therefore rapid, spectral acquisition of the Brillouin scattered light. Specifically, by exploiting a custom-built Fourier-transform imaging spectrometer and the symmetric properties of the Brillouin spectrum, we experimentally demonstrate full-field 2D spectral Brillouin imaging of phantoms as well as biological samples, at a throughput of up to 40,000 spectra per second over a ~300um field-of-view. This represents an approximately three orders of magnitude improvement in speed and throughput compared to standard confocal methods while retaining high spatial resolution and the capability to acquire three-dimensional images of photosensitive samples in biology and medicine. |
| title | Full-field Brillouin microscopy based on an imaging Fourier transform spectrometer |
| topic | Optics |
| url | https://arxiv.org/abs/2409.02092 |