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| Main Authors: | , , , |
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| Format: | Preprint |
| Published: |
2024
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2409.15080 |
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Table of Contents:
- We introduce a novel gene regulatory network (GRN) inference method that integrates optimal transport (OT) with a deep-learning structural inference model. Advances in next-generation sequencing enable detailed yet destructive gene expression assays at the single-cell level, resulting in the loss of cell evolutionary trajectories. Due to technological and cost constraints, single-cell experiments often feature cells sampled at irregular and sparse time points with a small sample size. Although trajectory-based structural inference models can accurately reveal the underlying interaction graph from observed data, their efficacy depends on the inputs of thousands of regularly sampled trajectories. The irregularly-sampled nature of single-cell data precludes the direct use of these powerful models for reconstructing GRNs. Optimal transport, a classical mathematical framework that minimize transportation costs between distributions, has shown promise in multi-omics data integration and cell fate prediction. Utilizing OT, our method constructs mappings between consecutively sampled cells to form cell-level trajectories, which are given as input to a structural inference model that recovers the GRN from single-cell data. Through case studies in two synthetic datasets, we demonstrate the feasibility of our proposed method and its promising performance over eight state-of-the-art GRN inference methods.