Increasing the spatial bandwidth product in Light Field Microscopy with remote scanning
Fuente:
arXiv
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| Autori principali: | , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| _version_ | 1866929535701745664 |
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| author | Bazin, Aymerick Badon, Amaury |
| author_facet | Bazin, Aymerick Badon, Amaury |
| contents | Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy, incorporating a motorized tilting mirror upstream the camera. Depending on the scanning pattern, the field of view and/or the lateral resolution can be greatly improved. Our microscope technique is simple, versatile and configured for both bright-field and epifluorescence modes. We demonstrate its performances with imaging of multi-cellular aggregates of various shape and sizes. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2410_07975 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Increasing the spatial bandwidth product in Light Field Microscopy with remote scanning Bazin, Aymerick Badon, Amaury Optics Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy, incorporating a motorized tilting mirror upstream the camera. Depending on the scanning pattern, the field of view and/or the lateral resolution can be greatly improved. Our microscope technique is simple, versatile and configured for both bright-field and epifluorescence modes. We demonstrate its performances with imaging of multi-cellular aggregates of various shape and sizes. |
| title | Increasing the spatial bandwidth product in Light Field Microscopy with remote scanning |
| topic | Optics |
| url | https://arxiv.org/abs/2410.07975 |