Effect of Temperature and TiO$_{2}$ NP Concentration on DNA Stability under Conditions Close to Physiological Ones
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arXiv
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| Autori principali: | , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| _version_ | 1866909356682903552 |
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| author | Usenko, Evgeniya Svidzerska, Anastasiia Glamazda, Alexander Valeev, Vladimir Laguta, Anna Karachevtsev, Victor |
| author_facet | Usenko, Evgeniya Svidzerska, Anastasiia Glamazda, Alexander Valeev, Vladimir Laguta, Anna Karachevtsev, Victor |
| contents | The present work is devoted to the study of the thermostability of native DNA during binding to TiO$_{2}$ nanoparticles (NPs) at various concentrations under conditions close to physiological (0.1 M Na $^+$, pH 7) using thermal denaturation method and dynamic light scattering (DLS). The analysis of the DNA melting curves in the presence of TiO$_{2}$ NPs revealed a temperature range in which the light absorption of DNA decreases. This is explained by the formation of an ordered structure of partially unwound DNA strands on the NP surface. The DNA:TiO$_{2}$ NP nanoassemblies formed at the initial stages remained stable in the wide temperature range. The performed temperature-dependent DLS measurements of the DNA:TiO$_{2}$ NP suspension at pH 7 have not revealed the aggregation of the DNA:TiO$_{2}$ NP nanoassemblies that had been observed at pH 5. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2410_15197 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Effect of Temperature and TiO$_{2}$ NP Concentration on DNA Stability under Conditions Close to Physiological Ones Usenko, Evgeniya Svidzerska, Anastasiia Glamazda, Alexander Valeev, Vladimir Laguta, Anna Karachevtsev, Victor Biological Physics The present work is devoted to the study of the thermostability of native DNA during binding to TiO$_{2}$ nanoparticles (NPs) at various concentrations under conditions close to physiological (0.1 M Na $^+$, pH 7) using thermal denaturation method and dynamic light scattering (DLS). The analysis of the DNA melting curves in the presence of TiO$_{2}$ NPs revealed a temperature range in which the light absorption of DNA decreases. This is explained by the formation of an ordered structure of partially unwound DNA strands on the NP surface. The DNA:TiO$_{2}$ NP nanoassemblies formed at the initial stages remained stable in the wide temperature range. The performed temperature-dependent DLS measurements of the DNA:TiO$_{2}$ NP suspension at pH 7 have not revealed the aggregation of the DNA:TiO$_{2}$ NP nanoassemblies that had been observed at pH 5. |
| title | Effect of Temperature and TiO$_{2}$ NP Concentration on DNA Stability under Conditions Close to Physiological Ones |
| topic | Biological Physics |
| url | https://arxiv.org/abs/2410.15197 |