Dynamic Spectral fluorescence microscopy via Event-based & CMOS image-sensor fusion
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arXiv
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| Format: | Preprint |
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2024
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| _version_ | 1866912079128035328 |
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| author | Baird, Richard G. Majumder, Apratim Menon, Rajesh |
| author_facet | Baird, Richard G. Majumder, Apratim Menon, Rajesh |
| contents | We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of $\sim10\thinspaceμ$s ($\sim\thinspace$50,000 frames/s), while the CIS provides diffraction-limited spatial resolution. A diffractive optical element encodes spectral information into a diffractogram, which is recorded by the CIS. The diffractogram is processed using a deep neural network to resolve the fluorescence of two beads, whose emission peaks are separated by only 7 nm and exhibit an 88\% spectral overlap. We validate our microscope by imaging the capillary flow of fluorescent beads, demonstrating a significant advancement in ultra-fast spectral microscopy. This technique holds broad potential for elucidating foundational dynamic biological processes. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2410_15552 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Dynamic Spectral fluorescence microscopy via Event-based & CMOS image-sensor fusion Baird, Richard G. Majumder, Apratim Menon, Rajesh Optics We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of $\sim10\thinspaceμ$s ($\sim\thinspace$50,000 frames/s), while the CIS provides diffraction-limited spatial resolution. A diffractive optical element encodes spectral information into a diffractogram, which is recorded by the CIS. The diffractogram is processed using a deep neural network to resolve the fluorescence of two beads, whose emission peaks are separated by only 7 nm and exhibit an 88\% spectral overlap. We validate our microscope by imaging the capillary flow of fluorescent beads, demonstrating a significant advancement in ultra-fast spectral microscopy. This technique holds broad potential for elucidating foundational dynamic biological processes. |
| title | Dynamic Spectral fluorescence microscopy via Event-based & CMOS image-sensor fusion |
| topic | Optics |
| url | https://arxiv.org/abs/2410.15552 |