A Fluorescent Ratiometric Potassium Sensor Based on IPG4-Silica Microparticles for Selective Detection and Fluorescence Imaging of Potassium Cations
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| Main Authors: | , , , , , , |
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| Format: | Preprint |
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2024
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| _version_ | 1866913561064767488 |
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| author | Colella, Francesco Forciniti, Stefania Onesto, Valentina Grasso, Giuliana Iuele, Helena Gigli, Giuseppe del Mercato, Loretta L. |
| author_facet | Colella, Francesco Forciniti, Stefania Onesto, Valentina Grasso, Giuliana Iuele, Helena Gigli, Giuseppe del Mercato, Loretta L. |
| contents | Potassium cations play many important roles in living organisms, especially in electro-physiology, since they are involved in neurotransmission and muscle contractions. We report the synthesis of a ratiometric fluorescent microsensor for potassium (K+) detection, based on the fluorescent probe ION potassium green 4. Potassium-sensitive fluorescent microparticles were obtained by using silica as core material. We obtained silica-based microsensors with a size in the micrometer range, spherical shape, good monodispersity, optimal selectivity and a sensitivity range between 0 to 40 mM. The microsensors also proved to be non-toxic in cell cultures as well as suitable for fluorescent imaging, offering new possibilities for non-invasive optical detection, quantification and in situ monitoring of K+ variations in cell culture systems. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2410_17795 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | A Fluorescent Ratiometric Potassium Sensor Based on IPG4-Silica Microparticles for Selective Detection and Fluorescence Imaging of Potassium Cations Colella, Francesco Forciniti, Stefania Onesto, Valentina Grasso, Giuliana Iuele, Helena Gigli, Giuseppe del Mercato, Loretta L. Biological Physics Chemical Physics Potassium cations play many important roles in living organisms, especially in electro-physiology, since they are involved in neurotransmission and muscle contractions. We report the synthesis of a ratiometric fluorescent microsensor for potassium (K+) detection, based on the fluorescent probe ION potassium green 4. Potassium-sensitive fluorescent microparticles were obtained by using silica as core material. We obtained silica-based microsensors with a size in the micrometer range, spherical shape, good monodispersity, optimal selectivity and a sensitivity range between 0 to 40 mM. The microsensors also proved to be non-toxic in cell cultures as well as suitable for fluorescent imaging, offering new possibilities for non-invasive optical detection, quantification and in situ monitoring of K+ variations in cell culture systems. |
| title | A Fluorescent Ratiometric Potassium Sensor Based on IPG4-Silica Microparticles for Selective Detection and Fluorescence Imaging of Potassium Cations |
| topic | Biological Physics Chemical Physics |
| url | https://arxiv.org/abs/2410.17795 |