Radical-mediated Electrical Enzyme Assay For At-home Clinical Test
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arXiv
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| Autori principali: | , , , , , , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| _version_ | 1866912105988358144 |
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| author | Jang, Hyun-June Joung, Hyou-Arm Shi, Xiaoao Ding, Rui Wagner, Justine Tang, Erting Zhuang, Wen Ryu, Byunghoon Chen, Guanmin Yeo, Kiang-Teck Jerry Huang, Jun Chen, Junhong |
| author_facet | Jang, Hyun-June Joung, Hyou-Arm Shi, Xiaoao Ding, Rui Wagner, Justine Tang, Erting Zhuang, Wen Ryu, Byunghoon Chen, Guanmin Yeo, Kiang-Teck Jerry Huang, Jun Chen, Junhong |
| contents | To meet the growing demand for accurate, rapid, and cost-effective at-home clinical testing, we developed a radical-mediated enzyme assay (REEA) integrated with a paper fluidic system and electrically read by a handheld field-effect transistor (FET) device. The REEA utilizes horseradish peroxidase (HRP) to catalyze the conversion of aromatic substrates into radical forms, producing protons detected by an ion-sensitive FET for biomarker quantification. Through screening 14 phenolic compounds, halogenated phenols emerged as optimal substrates for the REEA. Encased in an affordable cartridge ($0.55 per test), the system achieved a detection limit of 146 fg/mL for estradiol (E2), with a coefficient of variation (CV) below 9.2% in E2-spiked samples and an r2 of 0.963 across a measuring range of 19 to 4,551 pg/mL in clinical plasma samples, providing results in under 10 minutes. This adaptable system not only promises to offer a fast and reliable platform, but also holds significant potential for expansion to a wide array of biomarkers, paving the way for broader clinical and home-based applications. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2411_03111 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Radical-mediated Electrical Enzyme Assay For At-home Clinical Test Jang, Hyun-June Joung, Hyou-Arm Shi, Xiaoao Ding, Rui Wagner, Justine Tang, Erting Zhuang, Wen Ryu, Byunghoon Chen, Guanmin Yeo, Kiang-Teck Jerry Huang, Jun Chen, Junhong Quantitative Methods To meet the growing demand for accurate, rapid, and cost-effective at-home clinical testing, we developed a radical-mediated enzyme assay (REEA) integrated with a paper fluidic system and electrically read by a handheld field-effect transistor (FET) device. The REEA utilizes horseradish peroxidase (HRP) to catalyze the conversion of aromatic substrates into radical forms, producing protons detected by an ion-sensitive FET for biomarker quantification. Through screening 14 phenolic compounds, halogenated phenols emerged as optimal substrates for the REEA. Encased in an affordable cartridge ($0.55 per test), the system achieved a detection limit of 146 fg/mL for estradiol (E2), with a coefficient of variation (CV) below 9.2% in E2-spiked samples and an r2 of 0.963 across a measuring range of 19 to 4,551 pg/mL in clinical plasma samples, providing results in under 10 minutes. This adaptable system not only promises to offer a fast and reliable platform, but also holds significant potential for expansion to a wide array of biomarkers, paving the way for broader clinical and home-based applications. |
| title | Radical-mediated Electrical Enzyme Assay For At-home Clinical Test |
| topic | Quantitative Methods |
| url | https://arxiv.org/abs/2411.03111 |