Computational refocusing in phase-resolved confocal microscopy
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arXiv
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| Hauptverfasser: | , , , , , |
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| Format: | Preprint |
| Veröffentlicht: |
2024
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| _version_ | 1866912116291665920 |
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| author | Schnell, Martin King, Melanie Buercklin, Sam Sarriugarte, Paulo Hillenbrand, Rainer Carney, P. Scott |
| author_facet | Schnell, Martin King, Melanie Buercklin, Sam Sarriugarte, Paulo Hillenbrand, Rainer Carney, P. Scott |
| contents | We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic holography consistent with the wave physics and the parameters of the microscope. An experimental demonstration is shown to restore an in-focus image of a test object from data acquired at several focal plane off-sets. Numerical refocusing can provide focused views on samples with large height variation with a potential application in confocal optical surface profiling. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2411_07865 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Computational refocusing in phase-resolved confocal microscopy Schnell, Martin King, Melanie Buercklin, Sam Sarriugarte, Paulo Hillenbrand, Rainer Carney, P. Scott Optics We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic holography consistent with the wave physics and the parameters of the microscope. An experimental demonstration is shown to restore an in-focus image of a test object from data acquired at several focal plane off-sets. Numerical refocusing can provide focused views on samples with large height variation with a potential application in confocal optical surface profiling. |
| title | Computational refocusing in phase-resolved confocal microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2411.07865 |