Flat Cell Imaging
Fuente:
arXiv
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| Auteurs principaux: | , , |
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| Format: | Preprint |
| Publié: |
2024
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| _version_ | 1866916487979073536 |
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| author | Nasirimarekani, Vahid Ditte, Zuzana Bodenschatz, Eberhard |
| author_facet | Nasirimarekani, Vahid Ditte, Zuzana Bodenschatz, Eberhard |
| contents | Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for measuring the spatio-temporal dynamics of cellular components. One solution to this issue is expansion microscopy, which cannot be used for living cells. Here, we present a method for flattening live cells to a thickness of down to 200 nanometers by confining them between two surface-treated transparent plates. The anti-fouling coating on the surfaces restricts the cells to a quasi-two-dimensional space by exerting osmotic control and preventing surface adhesion. This technique increases the distance between cellular components, thereby enabling high-resolution imaging of their spatio-temporal dynamics. The viability and phenotype of various cell types are demonstrated to be unaltered upon release from flat-cell confinement. The flat cell imaging method is a robust and straightforward technique, making it a practical choice for optical microscopy. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2411_12656 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Flat Cell Imaging Nasirimarekani, Vahid Ditte, Zuzana Bodenschatz, Eberhard Optics Quantitative Methods Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for measuring the spatio-temporal dynamics of cellular components. One solution to this issue is expansion microscopy, which cannot be used for living cells. Here, we present a method for flattening live cells to a thickness of down to 200 nanometers by confining them between two surface-treated transparent plates. The anti-fouling coating on the surfaces restricts the cells to a quasi-two-dimensional space by exerting osmotic control and preventing surface adhesion. This technique increases the distance between cellular components, thereby enabling high-resolution imaging of their spatio-temporal dynamics. The viability and phenotype of various cell types are demonstrated to be unaltered upon release from flat-cell confinement. The flat cell imaging method is a robust and straightforward technique, making it a practical choice for optical microscopy. |
| title | Flat Cell Imaging |
| topic | Optics Quantitative Methods |
| url | https://arxiv.org/abs/2411.12656 |