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| Autori principali: | , , , , , , , , , |
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| Natura: | Preprint |
| Pubblicazione: |
2024
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| Soggetti: | |
| Accesso online: | https://arxiv.org/abs/2411.17158 |
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| _version_ | 1866913585970544640 |
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| author | Zhang, Rongrong Wan, Shengjie Xiong, Jiarui Ni, Lei Li, Ye Huang, Yajia Li, Bing Li, Mei Yang, Shuai Jin, Fan |
| author_facet | Zhang, Rongrong Wan, Shengjie Xiong, Jiarui Ni, Lei Li, Ye Huang, Yajia Li, Bing Li, Mei Yang, Shuai Jin, Fan |
| contents | Natural biological systems process environmental information through both amplitude and frequency-modulated signals, yet engineered biological circuits have largely relied on amplitude-based regulation alone. Despite the prevalence of frequency-encoded signals in natural systems, fundamental challenges in designing and implementing frequency-responsive gene circuits have limited their development in synthetic biology. Here we present a Time-Resolved Gene Circuit (TRGC) architecture that enables frequency-to-amplitude signal conversion in engineered biological systems. Through systematic analysis, we establish a theoretical framework that guides the design of synthetic circuits capable of distinct frequency-dependent responses, implementing both high-pass and low-pass filtering behaviors. To enable rigorous characterization of these dynamic circuits, we developed a high-throughput automated platform that ensures stable and reproducible measurements of frequency-dependent r esponses across diverse conditions. Using this platform, we demonstrate that these frequency-modulated circuits can access cellular states unreachable through conventional amplitude modulation, significantly expanding the controllable gene expression space in multi-gene systems. Our results show that frequency modulation expands the range of achievable expression patterns when controlling multiple genes through a single input, demonstrating a new paradigm for engineering cellular behaviors. This work establishes frequency modulation as a powerful strategy for expanding the capabilities of engineered biological systems and enhancing cellular response to dynamic signals. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2411_17158 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Synthetic frequency-controlled gene circuits unlock expanded cellular states Zhang, Rongrong Wan, Shengjie Xiong, Jiarui Ni, Lei Li, Ye Huang, Yajia Li, Bing Li, Mei Yang, Shuai Jin, Fan Biological Physics Molecular Networks Natural biological systems process environmental information through both amplitude and frequency-modulated signals, yet engineered biological circuits have largely relied on amplitude-based regulation alone. Despite the prevalence of frequency-encoded signals in natural systems, fundamental challenges in designing and implementing frequency-responsive gene circuits have limited their development in synthetic biology. Here we present a Time-Resolved Gene Circuit (TRGC) architecture that enables frequency-to-amplitude signal conversion in engineered biological systems. Through systematic analysis, we establish a theoretical framework that guides the design of synthetic circuits capable of distinct frequency-dependent responses, implementing both high-pass and low-pass filtering behaviors. To enable rigorous characterization of these dynamic circuits, we developed a high-throughput automated platform that ensures stable and reproducible measurements of frequency-dependent r esponses across diverse conditions. Using this platform, we demonstrate that these frequency-modulated circuits can access cellular states unreachable through conventional amplitude modulation, significantly expanding the controllable gene expression space in multi-gene systems. Our results show that frequency modulation expands the range of achievable expression patterns when controlling multiple genes through a single input, demonstrating a new paradigm for engineering cellular behaviors. This work establishes frequency modulation as a powerful strategy for expanding the capabilities of engineered biological systems and enhancing cellular response to dynamic signals. |
| title | Synthetic frequency-controlled gene circuits unlock expanded cellular states |
| topic | Biological Physics Molecular Networks |
| url | https://arxiv.org/abs/2411.17158 |