Cargo Delivery to Cells Using Laser-Irradiated Carbon-Black-Loaded PDMS
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arXiv
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| Format: | Preprint |
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2024
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| _version_ | 1866909413065883648 |
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| author | Shen, Weilu Chen, Anqi Paink, Gurminder K. Black, Nicole Weitz, David Mazur, Eric |
| author_facet | Shen, Weilu Chen, Anqi Paink, Gurminder K. Black, Nicole Weitz, David Mazur, Eric |
| contents | Effective intracellular delivery is essential for successful gene editing of cells. Spatially selective delivery to cells that is simultaneously precise, consistent, and non-destructive remains challenging using conventional state-of-the-art techniques. Here, we introduce a carrier-free method for spatiotemporal delivery of fluorescently labeled cargo into both adherent and suspension cells using carbon-black-embedded polydimethylsiloxane (PDMS) substrates irradiated by nanosecond laser pulses. This low-cost, biocompatible material, coupled with an optical approach, enables scalable, spatially selective, and sequential delivery of multiple cargo molecules, including FITC-dextran and siRNA, to a broad range of cells. Notably, we achieved siRNA delivery into the cytoplasm of hard-to-transfect K562 cells with 45% efficiency, while maintaining nearly 100% cell viability. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2412_02032 |
| institution | arXiv |
| publishDate | 2024 |
| record_format | arxiv |
| spellingShingle | Cargo Delivery to Cells Using Laser-Irradiated Carbon-Black-Loaded PDMS Shen, Weilu Chen, Anqi Paink, Gurminder K. Black, Nicole Weitz, David Mazur, Eric Materials Science Biological Physics Effective intracellular delivery is essential for successful gene editing of cells. Spatially selective delivery to cells that is simultaneously precise, consistent, and non-destructive remains challenging using conventional state-of-the-art techniques. Here, we introduce a carrier-free method for spatiotemporal delivery of fluorescently labeled cargo into both adherent and suspension cells using carbon-black-embedded polydimethylsiloxane (PDMS) substrates irradiated by nanosecond laser pulses. This low-cost, biocompatible material, coupled with an optical approach, enables scalable, spatially selective, and sequential delivery of multiple cargo molecules, including FITC-dextran and siRNA, to a broad range of cells. Notably, we achieved siRNA delivery into the cytoplasm of hard-to-transfect K562 cells with 45% efficiency, while maintaining nearly 100% cell viability. |
| title | Cargo Delivery to Cells Using Laser-Irradiated Carbon-Black-Loaded PDMS |
| topic | Materials Science Biological Physics |
| url | https://arxiv.org/abs/2412.02032 |