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Main Authors: Kamseu-Mogo, Jean-Paul, Soulier, Manon, Kamgang-Youbi, Georges, Mafouasson, Hortense Noëlle Apala, Dufour, Thierry
Format: Preprint
Published: 2024
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Online Access:https://arxiv.org/abs/2412.09759
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author Kamseu-Mogo, Jean-Paul
Soulier, Manon
Kamgang-Youbi, Georges
Mafouasson, Hortense Noëlle Apala
Dufour, Thierry
author_facet Kamseu-Mogo, Jean-Paul
Soulier, Manon
Kamgang-Youbi, Georges
Mafouasson, Hortense Noëlle Apala
Dufour, Thierry
contents In this study, we investigate the effects of pre-germinative and post-germinative plasma treatments, applied separately or in combination, to improve maize germination and early seedling development. Pre-germinative treatment consists of priming the seeds with a dry atmospheric plasma (DAP) generated by a dielectric barrier device (DBD), characterized by minimal radiative emission, low electrical power (4 W) and high emissions of O, OH and NO radicals. Post-germinative treatment, known as plasma-activated water (PAW), uses a single-pin electrode device (SPED) to generate a DC discharge that features a power of 126 W and produces large amounts of OH radicals. The resulting PAW, after 5 minutes of SPED treatment, induces a slight acidification and increased concentrations of nitrate ions (from 24 to 250 mg/L), nitrite ions (from less than 0.1 to 56.1 mg/L) and hydrogen peroxide (from 0.3 to 18.5 mg/L). Results indicate that DAP applied on maize seeds for 20 min boosts their germination rate up to 90% (versus only 65% for untreated seeds) while reducing the median germination time by 37.5%. Then, seedling growth monitoring is achieved on control, DAP, PAW and DAP+PAW groups to assess stem length, hypocotyl length, leaf count, collar diameter and fresh/dry mass. The DAP+PAW group shows the most robust growth, demonstrating a synergistic effect of the combined treatments, particularly with significantly longer stem lengths. Additionally, physiological analyses of seedling leaves indicate a decrease in chlorophyll content despite enhanced growth, while fluorescence microscopy reveals a reduction in stomatal density in leaves treated with DAP and PAW, especially in the combined treatment group, potentially impacting photosynthetic efficiency and water regulation.
format Preprint
id arxiv_https___arxiv_org_abs_2412_09759
institution arXiv
publishDate 2024
record_format arxiv
spellingShingle Advancements in Maize Cultivation: Synergistic Effects of Dry Atmospheric Plasma Combined with Plasma-Activated Water
Kamseu-Mogo, Jean-Paul
Soulier, Manon
Kamgang-Youbi, Georges
Mafouasson, Hortense Noëlle Apala
Dufour, Thierry
Plasma Physics
In this study, we investigate the effects of pre-germinative and post-germinative plasma treatments, applied separately or in combination, to improve maize germination and early seedling development. Pre-germinative treatment consists of priming the seeds with a dry atmospheric plasma (DAP) generated by a dielectric barrier device (DBD), characterized by minimal radiative emission, low electrical power (4 W) and high emissions of O, OH and NO radicals. Post-germinative treatment, known as plasma-activated water (PAW), uses a single-pin electrode device (SPED) to generate a DC discharge that features a power of 126 W and produces large amounts of OH radicals. The resulting PAW, after 5 minutes of SPED treatment, induces a slight acidification and increased concentrations of nitrate ions (from 24 to 250 mg/L), nitrite ions (from less than 0.1 to 56.1 mg/L) and hydrogen peroxide (from 0.3 to 18.5 mg/L). Results indicate that DAP applied on maize seeds for 20 min boosts their germination rate up to 90% (versus only 65% for untreated seeds) while reducing the median germination time by 37.5%. Then, seedling growth monitoring is achieved on control, DAP, PAW and DAP+PAW groups to assess stem length, hypocotyl length, leaf count, collar diameter and fresh/dry mass. The DAP+PAW group shows the most robust growth, demonstrating a synergistic effect of the combined treatments, particularly with significantly longer stem lengths. Additionally, physiological analyses of seedling leaves indicate a decrease in chlorophyll content despite enhanced growth, while fluorescence microscopy reveals a reduction in stomatal density in leaves treated with DAP and PAW, especially in the combined treatment group, potentially impacting photosynthetic efficiency and water regulation.
title Advancements in Maize Cultivation: Synergistic Effects of Dry Atmospheric Plasma Combined with Plasma-Activated Water
topic Plasma Physics
url https://arxiv.org/abs/2412.09759