High-Resolution Imaging of Plant Delayed Luminescence
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arXiv
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| Auteurs principaux: | , , , , , , , |
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| Format: | Preprint |
| Publié: |
2025
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| _version_ | 1866916912552738816 |
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| author | Liu, Yan-Xia Fan, Hai-Yu Wang, Yu-Hao Wang, Yan-Liang Li, Sheng-Wen Li, Shi-Jian Yao, Xu-Ri Zhao, Qing |
| author_facet | Liu, Yan-Xia Fan, Hai-Yu Wang, Yu-Hao Wang, Yan-Liang Li, Sheng-Wen Li, Shi-Jian Yao, Xu-Ri Zhao, Qing |
| contents | Delayed luminescence (DL) is a quantized signal that is characteristic of photoexcited molecules entering a relaxed state. Studying DL provides critical insight into photophysical mechanisms through the analysis of specific spatiotemporal dynamics. In this study, we developed a high-sensitivity DL imaging system using a quantitative scientific complementary metal-oxide-semiconductor (qCMOS) camera and a single-photon counting resolution. By optimizing the optical architecture and signal processing algorithms together, we achieved full-field spatiotemporal DL imaging at megapixel resolution (i.e., $2304 \times 4096$ pixels). Key findings include the following: (1) we observed spatial heterogeneity in DL intensity across the leaves of Arabidopsis thaliana, with stronger signals detected in veins and at sites of mechanical injury; (2) species-specific DL responses occur in response to oxidative stress, with Hydrocotyle vulgaris and Ginkgo biloba showing enhanced central DL activity; (3) excitation using white light induced maximum DL intensity, while red and blue light differentially modulated decay kinetics. Finally, we develop a two-level quantum model that links DL dynamics to the populations of excited-state electrons, thereby developing a theoretical framework for future photophysical research. Collectively, this work establishes a theoretical and technological framework for advancing plant phenotyping under stress conditions and optimizing light environments. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2501_01173 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | High-Resolution Imaging of Plant Delayed Luminescence Liu, Yan-Xia Fan, Hai-Yu Wang, Yu-Hao Wang, Yan-Liang Li, Sheng-Wen Li, Shi-Jian Yao, Xu-Ri Zhao, Qing Optics Biological Physics Medical Physics Delayed luminescence (DL) is a quantized signal that is characteristic of photoexcited molecules entering a relaxed state. Studying DL provides critical insight into photophysical mechanisms through the analysis of specific spatiotemporal dynamics. In this study, we developed a high-sensitivity DL imaging system using a quantitative scientific complementary metal-oxide-semiconductor (qCMOS) camera and a single-photon counting resolution. By optimizing the optical architecture and signal processing algorithms together, we achieved full-field spatiotemporal DL imaging at megapixel resolution (i.e., $2304 \times 4096$ pixels). Key findings include the following: (1) we observed spatial heterogeneity in DL intensity across the leaves of Arabidopsis thaliana, with stronger signals detected in veins and at sites of mechanical injury; (2) species-specific DL responses occur in response to oxidative stress, with Hydrocotyle vulgaris and Ginkgo biloba showing enhanced central DL activity; (3) excitation using white light induced maximum DL intensity, while red and blue light differentially modulated decay kinetics. Finally, we develop a two-level quantum model that links DL dynamics to the populations of excited-state electrons, thereby developing a theoretical framework for future photophysical research. Collectively, this work establishes a theoretical and technological framework for advancing plant phenotyping under stress conditions and optimizing light environments. |
| title | High-Resolution Imaging of Plant Delayed Luminescence |
| topic | Optics Biological Physics Medical Physics |
| url | https://arxiv.org/abs/2501.01173 |