Spatially Multiplexed Interferometric Microscopy with one-dimensional diffraction grating
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arXiv
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| Format: | Preprint |
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2025
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| _version_ | 1866917894928990208 |
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| author | Picazo-Bueno, Jose Angel Trusiak, Maciej Garcia, Javier Mico, Vicente |
| author_facet | Picazo-Bueno, Jose Angel Trusiak, Maciej Garcia, Javier Mico, Vicente |
| contents | Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy (SMIM) is a DHM technique that implements a common-path interferometric layout in the embodiment of a standard microscope to achieve QPI. More concretely, SMIM introduces three minimal modifications: 1) replaces the broadband illumination of the microscope by a coherent or partially coherent light source, 2) divides the input plane into two or three regions for transmission in parallel of both imaging and reference beams, and 3) includes a one-dimensional diffraction grating or a beam splitter cube for holographic recording. Hence, SMIM is a cost-effective, extremely simple, and highly stable manner of converting a standard bright field microscope into a holographic one. The goal of this contribution is to provide a review of the SMIM approaches implemented using a one-dimensional (1D) diffraction grating, and highlight vast range of capabilities including super-resolved, reflective, transflective, noise-reduced and single-shot slightly off-axis amplitude and phase imaging. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2501_10023 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Spatially Multiplexed Interferometric Microscopy with one-dimensional diffraction grating Picazo-Bueno, Jose Angel Trusiak, Maciej Garcia, Javier Mico, Vicente Optics Digital holographic microscopy (DHM) applied to quantitative phase imaging (QPI) has been successfully demonstrated as a powerful label-free method to analyse the optical properties of cells. Spatially multiplexed interferometric microscopy (SMIM) is a DHM technique that implements a common-path interferometric layout in the embodiment of a standard microscope to achieve QPI. More concretely, SMIM introduces three minimal modifications: 1) replaces the broadband illumination of the microscope by a coherent or partially coherent light source, 2) divides the input plane into two or three regions for transmission in parallel of both imaging and reference beams, and 3) includes a one-dimensional diffraction grating or a beam splitter cube for holographic recording. Hence, SMIM is a cost-effective, extremely simple, and highly stable manner of converting a standard bright field microscope into a holographic one. The goal of this contribution is to provide a review of the SMIM approaches implemented using a one-dimensional (1D) diffraction grating, and highlight vast range of capabilities including super-resolved, reflective, transflective, noise-reduced and single-shot slightly off-axis amplitude and phase imaging. |
| title | Spatially Multiplexed Interferometric Microscopy with one-dimensional diffraction grating |
| topic | Optics |
| url | https://arxiv.org/abs/2501.10023 |