Incoherent lateral shearing digital holographic microscopy
Fuente:
arXiv
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| Autori principali: | , |
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| Natura: | Preprint |
| Pubblicazione: |
2025
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| _version_ | 1866913656559632384 |
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| author | Běhal, Jaromír Ježek, Miroslav |
| author_facet | Běhal, Jaromír Ježek, Miroslav |
| contents | The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce a novel incoherent quantitative phase imaging technique based on lateral shearing digital holographic microscopy, with shearing distances exceeding the coherence area of the employed illumination -- a regime not previously achieved and exploited. This strategy consequently assures artifacts-free, high-accuracy, and high-resolution quantitative phase reconstructions. This regime extends the single-shot lateral shearing digital holographic microscopy towards incoherent illumination, thus increasing the space-time bandwidth product of the method. The practical common-path geometry also provides enhanced vibration resistance, which is essential for consistent time-lapse measurements. We verify the scalability and effectiveness of the method by investigating samples via multiple condenser-objective pairs, providing a wide range of lateral resolutions and fields of view, thereby assuring its applicability for various microscope settings. We experimentally verify long-term phase stability and unprecedented phase-reconstruction accuracy. Finally, we use our method for investigation of biological samples, including cheek cells, diatoms, and yeast cells, highlighting its potential for dynamic label-free analysis at the organelle level. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2501_11343 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Incoherent lateral shearing digital holographic microscopy Běhal, Jaromír Ježek, Miroslav Optics The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce a novel incoherent quantitative phase imaging technique based on lateral shearing digital holographic microscopy, with shearing distances exceeding the coherence area of the employed illumination -- a regime not previously achieved and exploited. This strategy consequently assures artifacts-free, high-accuracy, and high-resolution quantitative phase reconstructions. This regime extends the single-shot lateral shearing digital holographic microscopy towards incoherent illumination, thus increasing the space-time bandwidth product of the method. The practical common-path geometry also provides enhanced vibration resistance, which is essential for consistent time-lapse measurements. We verify the scalability and effectiveness of the method by investigating samples via multiple condenser-objective pairs, providing a wide range of lateral resolutions and fields of view, thereby assuring its applicability for various microscope settings. We experimentally verify long-term phase stability and unprecedented phase-reconstruction accuracy. Finally, we use our method for investigation of biological samples, including cheek cells, diatoms, and yeast cells, highlighting its potential for dynamic label-free analysis at the organelle level. |
| title | Incoherent lateral shearing digital holographic microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2501.11343 |