Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution

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Hauptverfasser: Chen, Guo, Yuan, Yuhao, Ni, Hongli, Ding, Guangrui, Li, Mingsheng, Zhu, Yifan, Li, Deming, Zeng, Hongru, He, Hongjian, Guo, Zhongyue, Cheng, Ji-Xin, Yang, Chen
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Veröffentlicht: 2025
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author Chen, Guo
Yuan, Yuhao
Ni, Hongli
Ding, Guangrui
Li, Mingsheng
Zhu, Yifan
Li, Deming
Zeng, Hongru
He, Hongjian
Guo, Zhongyue
Cheng, Ji-Xin
Yang, Chen
author_facet Chen, Guo
Yuan, Yuhao
Ni, Hongli
Ding, Guangrui
Li, Mingsheng
Zhu, Yifan
Li, Deming
Zeng, Hongru
He, Hongjian
Guo, Zhongyue
Cheng, Ji-Xin
Yang, Chen
contents Nonradiative photothermal (PT) and photoacoustic (PA) processes have found widespread applications in imaging, stimulation, and therapy. Mapping the generation and propagation of PA and PT waves with resolution is important to elucidate how these fields interact with biological systems. To this end, we introduce spatial offset pump-probe imaging (SOPPI). By spatially offsetting the pump beam and the probe beam, SOPPI can image simultaneously PA and PT wave propagation with nanosecond temporal resolution, micrometer spatial resolution, 65 MHz detection bandwidth, and a sensitivity of 9.9 Pa noise equivalent pressure. We first map the PA and PT evolution from a fiber emitter, and how the wave interacting with a mouse skull and brain slices. SOPPI imaging of PA waves from a tapered fiber with water as an absorber shows a wavelength-dependent generation, evanescent wave generated PA, and back-propagated acoustic Mach Cone. At last, a SOPPI-PACT is developed to reconstruct the pigment distribution inside a zebrafish larva with high precision and signal-to-noise ratio.
format Preprint
id arxiv_https___arxiv_org_abs_2502_03384
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution
Chen, Guo
Yuan, Yuhao
Ni, Hongli
Ding, Guangrui
Li, Mingsheng
Zhu, Yifan
Li, Deming
Zeng, Hongru
He, Hongjian
Guo, Zhongyue
Cheng, Ji-Xin
Yang, Chen
Optics
Applied Physics
Nonradiative photothermal (PT) and photoacoustic (PA) processes have found widespread applications in imaging, stimulation, and therapy. Mapping the generation and propagation of PA and PT waves with resolution is important to elucidate how these fields interact with biological systems. To this end, we introduce spatial offset pump-probe imaging (SOPPI). By spatially offsetting the pump beam and the probe beam, SOPPI can image simultaneously PA and PT wave propagation with nanosecond temporal resolution, micrometer spatial resolution, 65 MHz detection bandwidth, and a sensitivity of 9.9 Pa noise equivalent pressure. We first map the PA and PT evolution from a fiber emitter, and how the wave interacting with a mouse skull and brain slices. SOPPI imaging of PA waves from a tapered fiber with water as an absorber shows a wavelength-dependent generation, evanescent wave generated PA, and back-propagated acoustic Mach Cone. At last, a SOPPI-PACT is developed to reconstruct the pigment distribution inside a zebrafish larva with high precision and signal-to-noise ratio.
title Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution
topic Optics
Applied Physics
url https://arxiv.org/abs/2502.03384