Offset geometry for extended field-of-view in multi-contrast and multi-scale X-ray microtomography of lung cancer lobectomy specimens

Fuente: arXiv
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Autori principali: Allan, Harry, Doherty, Adam, Navarrete-León, Carlos, Morgó, Oriol Roche i, Jia, Yunpeng, Percival, Charlotte, Hagel, Zoe, Otter, Kate E J, Khaw, Chuen Ryan, Gowers, Kate, Hall, Helen, Janes, Sam M, Monk, Fleur, Moore, David, Jacob, Joseph, Endrizzi, Marco
Natura: Preprint
Pubblicazione: 2025
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author Allan, Harry
Doherty, Adam
Navarrete-León, Carlos
Morgó, Oriol Roche i
Jia, Yunpeng
Percival, Charlotte
Hagel, Zoe
Otter, Kate E J
Khaw, Chuen Ryan
Gowers, Kate
Hall, Helen
Janes, Sam M
Monk, Fleur
Moore, David
Jacob, Joseph
Endrizzi, Marco
author_facet Allan, Harry
Doherty, Adam
Navarrete-León, Carlos
Morgó, Oriol Roche i
Jia, Yunpeng
Percival, Charlotte
Hagel, Zoe
Otter, Kate E J
Khaw, Chuen Ryan
Gowers, Kate
Hall, Helen
Janes, Sam M
Monk, Fleur
Moore, David
Jacob, Joseph
Endrizzi, Marco
contents X-ray microtomography is a powerful non-destructive technique allowing 3D virtual histology of resected human tissue. The achievable imaging field-of-view, is however limited by the fixed number of detector elements, enforcing the requirement to sacrifice spatial resolution in order to image larger samples. In applications such as soft-tissue imaging, phase-contrast methods are often employed to enhance image contrast. Some of these methods, especially those suited to laboratory sources, rely on optical elements, the dimensions of which can impose a further limitation on the field-of-view. We describe an efficient method to double the maximum field-of-view of a cone-beam X-ray microtomography system, without sacrificing on spatial resolution, and including multi-contrast capabilities. We demonstrate an experimental realisation of the method, achieving exemplary reconstructions of a resected human lung sample, with a cubic voxel of 10.5 $μ$m linear dimensions, across a horizontal field-of-view of 4.3 cm. The same concepts are applied to free-space propagation imaging of a 2.7 mm segment of the same sample, achieving a cubic voxel of 450 nm linear dimensions. We show that the methodology can be applied at a range of different length-scales and geometries, and that it is directly compatible with complementary implementations of X-ray phase-contrast imaging.
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institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Offset geometry for extended field-of-view in multi-contrast and multi-scale X-ray microtomography of lung cancer lobectomy specimens
Allan, Harry
Doherty, Adam
Navarrete-León, Carlos
Morgó, Oriol Roche i
Jia, Yunpeng
Percival, Charlotte
Hagel, Zoe
Otter, Kate E J
Khaw, Chuen Ryan
Gowers, Kate
Hall, Helen
Janes, Sam M
Monk, Fleur
Moore, David
Jacob, Joseph
Endrizzi, Marco
Medical Physics
Instrumentation and Detectors
X-ray microtomography is a powerful non-destructive technique allowing 3D virtual histology of resected human tissue. The achievable imaging field-of-view, is however limited by the fixed number of detector elements, enforcing the requirement to sacrifice spatial resolution in order to image larger samples. In applications such as soft-tissue imaging, phase-contrast methods are often employed to enhance image contrast. Some of these methods, especially those suited to laboratory sources, rely on optical elements, the dimensions of which can impose a further limitation on the field-of-view. We describe an efficient method to double the maximum field-of-view of a cone-beam X-ray microtomography system, without sacrificing on spatial resolution, and including multi-contrast capabilities. We demonstrate an experimental realisation of the method, achieving exemplary reconstructions of a resected human lung sample, with a cubic voxel of 10.5 $μ$m linear dimensions, across a horizontal field-of-view of 4.3 cm. The same concepts are applied to free-space propagation imaging of a 2.7 mm segment of the same sample, achieving a cubic voxel of 450 nm linear dimensions. We show that the methodology can be applied at a range of different length-scales and geometries, and that it is directly compatible with complementary implementations of X-ray phase-contrast imaging.
title Offset geometry for extended field-of-view in multi-contrast and multi-scale X-ray microtomography of lung cancer lobectomy specimens
topic Medical Physics
Instrumentation and Detectors
url https://arxiv.org/abs/2502.10322