Revealing Microscopic Objects in Fluorescence Live Imaging by Video-to-video Translation Based on A Spatial-temporal Generative Adversarial Network
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arXiv
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| Autores principales: | , , |
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| Formato: | Preprint |
| Publicado: |
2025
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| _version_ | 1866915167437062144 |
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| author | Jiao, Yang Yang, Mei Weng, Mo |
| author_facet | Jiao, Yang Yang, Mei Weng, Mo |
| contents | In spite of being a valuable tool to simultaneously visualize multiple types of subcellular structures using spectrally distinct fluorescent labels, a standard fluoresce microscope is only able to identify a few microscopic objects; such a limit is largely imposed by the number of fluorescent labels available to the sample. In order to simultaneously visualize more objects, in this paper, we propose to use video-to-video translation that mimics the development process of microscopic objects. In essence, we use a microscopy video-to-video translation framework namely Spatial-temporal Generative Adversarial Network (STGAN) to reveal the spatial and temporal relationships between the microscopic objects, after which a microscopy video of one object can be translated to another object in a different domain. The experimental results confirm that the proposed STGAN is effective in microscopy video-to-video translation that mitigates the spectral conflicts caused by the limited fluorescent labels, allowing multiple microscopic objects be simultaneously visualized. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2502_16342 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Revealing Microscopic Objects in Fluorescence Live Imaging by Video-to-video Translation Based on A Spatial-temporal Generative Adversarial Network Jiao, Yang Yang, Mei Weng, Mo Image and Video Processing Computer Vision and Pattern Recognition In spite of being a valuable tool to simultaneously visualize multiple types of subcellular structures using spectrally distinct fluorescent labels, a standard fluoresce microscope is only able to identify a few microscopic objects; such a limit is largely imposed by the number of fluorescent labels available to the sample. In order to simultaneously visualize more objects, in this paper, we propose to use video-to-video translation that mimics the development process of microscopic objects. In essence, we use a microscopy video-to-video translation framework namely Spatial-temporal Generative Adversarial Network (STGAN) to reveal the spatial and temporal relationships between the microscopic objects, after which a microscopy video of one object can be translated to another object in a different domain. The experimental results confirm that the proposed STGAN is effective in microscopy video-to-video translation that mitigates the spectral conflicts caused by the limited fluorescent labels, allowing multiple microscopic objects be simultaneously visualized. |
| title | Revealing Microscopic Objects in Fluorescence Live Imaging by Video-to-video Translation Based on A Spatial-temporal Generative Adversarial Network |
| topic | Image and Video Processing Computer Vision and Pattern Recognition |
| url | https://arxiv.org/abs/2502.16342 |