Super-resolution Live-cell Fluorescence Lifetime Imaging
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arXiv
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| Main Authors: | , , , , , , , , , |
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| Format: | Preprint |
| Published: |
2025
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| _version_ | 1866915167997001728 |
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| author | Marchand, Raphaël Ortkrass, Henning Aziz, Daniel Pfanner, Franz Abbas, Eman Rizzoli, Silvio O. Hübner, Wolfgang Bowman, Adam Huser, Thomas Juffmann, Thomas |
| author_facet | Marchand, Raphaël Ortkrass, Henning Aziz, Daniel Pfanner, Franz Abbas, Eman Rizzoli, Silvio O. Hübner, Wolfgang Bowman, Adam Huser, Thomas Juffmann, Thomas |
| contents | Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM makes it readily compatible with live-cell imaging. Here, we combine SR-SIM with electro-optic fluorescence lifetime imaging (EOFLIM), adding the capability of monitoring physicochemical parameters with 156 nm spatial resolution at high frame rate for live-cell imaging. We demonstrate that our new SIMFLIM technique enables super-resolved multiplexed imaging of spectrally overlapping fluorophores, environmental sensing, and live-cell imaging. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2502_16672 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Super-resolution Live-cell Fluorescence Lifetime Imaging Marchand, Raphaël Ortkrass, Henning Aziz, Daniel Pfanner, Franz Abbas, Eman Rizzoli, Silvio O. Hübner, Wolfgang Bowman, Adam Huser, Thomas Juffmann, Thomas Optics Super-resolution Structured Illumination Microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM makes it readily compatible with live-cell imaging. Here, we combine SR-SIM with electro-optic fluorescence lifetime imaging (EOFLIM), adding the capability of monitoring physicochemical parameters with 156 nm spatial resolution at high frame rate for live-cell imaging. We demonstrate that our new SIMFLIM technique enables super-resolved multiplexed imaging of spectrally overlapping fluorophores, environmental sensing, and live-cell imaging. |
| title | Super-resolution Live-cell Fluorescence Lifetime Imaging |
| topic | Optics |
| url | https://arxiv.org/abs/2502.16672 |