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Main Authors: Chang, Yung-Ching, Liu, Chan-Chuan, Chan, Wan-Ping, Lin, Yu-Long, Sze, Chun-I, Chen, Shiuan-Yeh
Format: Preprint
Published: 2025
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Online Access:https://arxiv.org/abs/2503.05266
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author Chang, Yung-Ching
Liu, Chan-Chuan
Chan, Wan-Ping
Lin, Yu-Long
Sze, Chun-I
Chen, Shiuan-Yeh
author_facet Chang, Yung-Ching
Liu, Chan-Chuan
Chan, Wan-Ping
Lin, Yu-Long
Sze, Chun-I
Chen, Shiuan-Yeh
contents Glioblastoma (GBM) is an aggressive and fatal tumor. The infiltrative spread of GBM cells hinders the gross total resection. The residual GBM cells are significantly associated with survival and recurrence. Therefore, a theranostic method that can enhance the contrast between residual GBM and normal astrocyte (AS) cells as well as selectively eradicate GBM cells is highly desired. In this report, GBM and normal astrocyte cells are both cultured in the same microplate well to imitate a coexistence environment and treated with Raman tags functionalized by anti-EGFR. Compared to AS cells, GBM cells show 25% higher Raman emission, and their cell death rate increases by a factor of 2. These results demonstrate potential for selective eradication of the residual GBM cells guided by robust Raman signals after the primary GBM surgery.
format Preprint
id arxiv_https___arxiv_org_abs_2503_05266
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Selective Photothermal Eradication of Glioblastoma Cells Coexisting with Astrocytes by anti-EGFR Coated Raman Tags
Chang, Yung-Ching
Liu, Chan-Chuan
Chan, Wan-Ping
Lin, Yu-Long
Sze, Chun-I
Chen, Shiuan-Yeh
Applied Physics
Biological Physics
Glioblastoma (GBM) is an aggressive and fatal tumor. The infiltrative spread of GBM cells hinders the gross total resection. The residual GBM cells are significantly associated with survival and recurrence. Therefore, a theranostic method that can enhance the contrast between residual GBM and normal astrocyte (AS) cells as well as selectively eradicate GBM cells is highly desired. In this report, GBM and normal astrocyte cells are both cultured in the same microplate well to imitate a coexistence environment and treated with Raman tags functionalized by anti-EGFR. Compared to AS cells, GBM cells show 25% higher Raman emission, and their cell death rate increases by a factor of 2. These results demonstrate potential for selective eradication of the residual GBM cells guided by robust Raman signals after the primary GBM surgery.
title Selective Photothermal Eradication of Glioblastoma Cells Coexisting with Astrocytes by anti-EGFR Coated Raman Tags
topic Applied Physics
Biological Physics
url https://arxiv.org/abs/2503.05266