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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Preprint |
| Publié: |
2025
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| Accès en ligne: | https://arxiv.org/abs/2503.06123 |
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| _version_ | 1866917948449357824 |
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| author | Tang, Liya Qu, Qirui Long, Yiying Wu, Xia Liu, Jincan Xu, Ming Zhang, Hong Zhou, Lu Liu, Qiong Ai, Kun |
| author_facet | Tang, Liya Qu, Qirui Long, Yiying Wu, Xia Liu, Jincan Xu, Ming Zhang, Hong Zhou, Lu Liu, Qiong Ai, Kun |
| contents | Objectives This study aimed to elucidate the potential mechanisms of electroacupuncture (EA) in restoring detrusor-bladder neck dyssynergesia (DBND) following suprasacral spinal cord injury.
Methods A total of 52 adult female Sprague-Dawley rats were randomly assigned to either a sham group (n=12) or a spinal cord injury model group (n=40). In the model group, DBND was induced in 40 rats through Hassan Shaker spinal cord transection, with 24 rats surviving spinal shock and subsequently randomized into two groups: a model-only group (DBND, n=12) and an EA intervention group (DBND+EA, n=12). DBND+EA was administered at Ciliao (BL32), Zhongji (RN3), and Sanyinjiao (SP6) acupoints, for 20 minutes per session, once daily for 10 consecutive days. On day 29 post-injury, all rats underwent urodynamic assessments, followed by hematoxylin and eosin (HE) staining, tandem mass tag (TMT) proteomics, and Western blot (WB) analysis of the detrusor and bladder neck tissues.
Results Urodynamic evaluation demonstrated that EA intervention enhanced bladder function in DBND rats. HE staining indicated reduced fibroplasia in the detrusor muscle and alleviated inflammation in the bladder neck following EA. TMT proteomic analysis revealed 30 differentially expressed proteins (DEPs) in the detrusor and 59 DEPs in the bladder neck post-EA treatment. WB results corroborated these TMT findings.
Conclusion EA effectively promotes synergy between the detrusor muscle and bladder neck in DBND, likely by enhancing detrusor contractility and facilitating bladder neck relaxation during urination. This study provides mechanistic insights into the therapeutic role of EA in managing DBND. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2503_06123 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Mechanism of Electricacupuncture Treating Detrusor Bladder Neck Dyscoordination After Suprasacral Spinal Cord Injury by Proteomics Tang, Liya Qu, Qirui Long, Yiying Wu, Xia Liu, Jincan Xu, Ming Zhang, Hong Zhou, Lu Liu, Qiong Ai, Kun Biomolecules Objectives This study aimed to elucidate the potential mechanisms of electroacupuncture (EA) in restoring detrusor-bladder neck dyssynergesia (DBND) following suprasacral spinal cord injury. Methods A total of 52 adult female Sprague-Dawley rats were randomly assigned to either a sham group (n=12) or a spinal cord injury model group (n=40). In the model group, DBND was induced in 40 rats through Hassan Shaker spinal cord transection, with 24 rats surviving spinal shock and subsequently randomized into two groups: a model-only group (DBND, n=12) and an EA intervention group (DBND+EA, n=12). DBND+EA was administered at Ciliao (BL32), Zhongji (RN3), and Sanyinjiao (SP6) acupoints, for 20 minutes per session, once daily for 10 consecutive days. On day 29 post-injury, all rats underwent urodynamic assessments, followed by hematoxylin and eosin (HE) staining, tandem mass tag (TMT) proteomics, and Western blot (WB) analysis of the detrusor and bladder neck tissues. Results Urodynamic evaluation demonstrated that EA intervention enhanced bladder function in DBND rats. HE staining indicated reduced fibroplasia in the detrusor muscle and alleviated inflammation in the bladder neck following EA. TMT proteomic analysis revealed 30 differentially expressed proteins (DEPs) in the detrusor and 59 DEPs in the bladder neck post-EA treatment. WB results corroborated these TMT findings. Conclusion EA effectively promotes synergy between the detrusor muscle and bladder neck in DBND, likely by enhancing detrusor contractility and facilitating bladder neck relaxation during urination. This study provides mechanistic insights into the therapeutic role of EA in managing DBND. |
| title | Mechanism of Electricacupuncture Treating Detrusor Bladder Neck Dyscoordination After Suprasacral Spinal Cord Injury by Proteomics |
| topic | Biomolecules |
| url | https://arxiv.org/abs/2503.06123 |