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Main Authors: Barylli, Marcello, Saha, Joyaditya, Buffart, Tineke E., Koster, Jan, Lenos, Kristiaan J., Vermeulen, Louis, Sheraton, Vivek M.
Format: Preprint
Published: 2025
Subjects:
Online Access:https://arxiv.org/abs/2503.09568
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author Barylli, Marcello
Saha, Joyaditya
Buffart, Tineke E.
Koster, Jan
Lenos, Kristiaan J.
Vermeulen, Louis
Sheraton, Vivek M.
author_facet Barylli, Marcello
Saha, Joyaditya
Buffart, Tineke E.
Koster, Jan
Lenos, Kristiaan J.
Vermeulen, Louis
Sheraton, Vivek M.
contents Recent advances in single cell sequencing and multi-omics techniques have significantly improved our understanding of biological phenomena and our capacity to model them. Despite combined capture of data modalities showing similar progress, notably single cell transcriptomics and proteomics, simultaneous multi-omics level probing still remains challenging. As an alternative to combined capture of biological data, in this review, we explore current and upcoming methods for post-hoc network inference and integration with an emphasis on single cell transcriptomics and proteomics. By examining various approaches, from probabilistic models to graph-based algorithms, we outline the challenges and potential strategies for effectively combining biological data types while simultaneously highlighting the importance of model validation. With this review, we aim to inform readers of the breadth of tools currently available for the purpose-specific generation of heterogeneous multi-layer networks.
format Preprint
id arxiv_https___arxiv_org_abs_2503_09568
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Biological Multi-Layer and Single Cell Network-Based Multiomics Models - a Review
Barylli, Marcello
Saha, Joyaditya
Buffart, Tineke E.
Koster, Jan
Lenos, Kristiaan J.
Vermeulen, Louis
Sheraton, Vivek M.
Molecular Networks
Recent advances in single cell sequencing and multi-omics techniques have significantly improved our understanding of biological phenomena and our capacity to model them. Despite combined capture of data modalities showing similar progress, notably single cell transcriptomics and proteomics, simultaneous multi-omics level probing still remains challenging. As an alternative to combined capture of biological data, in this review, we explore current and upcoming methods for post-hoc network inference and integration with an emphasis on single cell transcriptomics and proteomics. By examining various approaches, from probabilistic models to graph-based algorithms, we outline the challenges and potential strategies for effectively combining biological data types while simultaneously highlighting the importance of model validation. With this review, we aim to inform readers of the breadth of tools currently available for the purpose-specific generation of heterogeneous multi-layer networks.
title Biological Multi-Layer and Single Cell Network-Based Multiomics Models - a Review
topic Molecular Networks
url https://arxiv.org/abs/2503.09568