Generalized reciprocal diffractive imaging for stand-alone, reference-free, fast-measurable quantitative phase microscopy
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arXiv
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| Auteurs principaux: | , , , |
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| Format: | Preprint |
| Publié: |
2025
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| _version_ | 1866912278438215680 |
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| author | Oh, Jeonghun Hugonnet, Herve Park, Weisun Park, YongKeun |
| author_facet | Oh, Jeonghun Hugonnet, Herve Park, Weisun Park, YongKeun |
| contents | Optical microscopy has been employed to derive salient characteristics of an object in various fields, including cell biology, flow cytometry, biopsy, and neuroscience. In particular, measuring the phase of light scattered from an object aroused great interest by allowing retrieving quantitative parameters such as refractive index, an intrinsic property of a material. Reciprocal diffractive imaging (RDI) has succeeded in recovering the light field scattered from diffusive objects without special restrictions on illumination and sample support from a single-shot intensity in the reference-free regime. However, RDI is limited to imaging samples in the diffusive regime, making application to biological samples difficult. Here, we extend RDI to biological applications by spatially filtering the transmitted fields in the pupil plane. The proposed method is demonstrated by imaging the objects with known structures and various biological samples, showing its capability as a stand-alone optical microscope. We believe that the presented advance could be at the forefront of quantitative phase imaging due to the unique advantages the technique possesses. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2503_13083 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Generalized reciprocal diffractive imaging for stand-alone, reference-free, fast-measurable quantitative phase microscopy Oh, Jeonghun Hugonnet, Herve Park, Weisun Park, YongKeun Optics Optical microscopy has been employed to derive salient characteristics of an object in various fields, including cell biology, flow cytometry, biopsy, and neuroscience. In particular, measuring the phase of light scattered from an object aroused great interest by allowing retrieving quantitative parameters such as refractive index, an intrinsic property of a material. Reciprocal diffractive imaging (RDI) has succeeded in recovering the light field scattered from diffusive objects without special restrictions on illumination and sample support from a single-shot intensity in the reference-free regime. However, RDI is limited to imaging samples in the diffusive regime, making application to biological samples difficult. Here, we extend RDI to biological applications by spatially filtering the transmitted fields in the pupil plane. The proposed method is demonstrated by imaging the objects with known structures and various biological samples, showing its capability as a stand-alone optical microscope. We believe that the presented advance could be at the forefront of quantitative phase imaging due to the unique advantages the technique possesses. |
| title | Generalized reciprocal diffractive imaging for stand-alone, reference-free, fast-measurable quantitative phase microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2503.13083 |