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Main Authors: Cao, Zhejian, Pandit, Santosh, Noa, Francoise M. Amombo, Zhang, Jian, Gao, Wengeng, Rahimi, Shadi, Öhrström, Lars, Mijakovic, Ivan
Format: Preprint
Published: 2025
Subjects:
Online Access:https://arxiv.org/abs/2503.16003
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author Cao, Zhejian
Pandit, Santosh
Noa, Francoise M. Amombo
Zhang, Jian
Gao, Wengeng
Rahimi, Shadi
Öhrström, Lars
Mijakovic, Ivan
author_facet Cao, Zhejian
Pandit, Santosh
Noa, Francoise M. Amombo
Zhang, Jian
Gao, Wengeng
Rahimi, Shadi
Öhrström, Lars
Mijakovic, Ivan
contents Mechano-bactericidal (MB) surfaces have been proposed as an emerging strategy for preventing biofilm formation. Unlike antibiotics and metal ions that chemically interfere with cellular processes, MB nanostructures cause physical damage to the bacteria. The antibacterial performance of artificial MB surfaces relies on rational control of surface features, which is difficult to achieve for large surfaces in real-life applications. Herein, we report a facile and scalable method for fabricating MB surfaces based on metal-organic frameworks (MOFs) using epitaxial MOF-on-MOF hybrids as building blocks with nanopillars of less than 5 nm tip diameter, 200 nm base diameter, and 300 nm length. Two methods of MOF surface assembly, in-situ growth and ex-situ dropcasting, result in surfaces with nanopillars in different orientations, both presenting MB actions (bactericidal efficiency of 83% for E. coli). Distinct MB mechanisms, including stretching, impaling, and apoptosis-like death induced by mechanical injury are discussed with the observed bacterial morphology on the obtained MOF surfaces.
format Preprint
id arxiv_https___arxiv_org_abs_2503_16003
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Mechano-Bactericidal Surfaces Achieved by Epitaxial Growth of Metal-Organic Frameworks
Cao, Zhejian
Pandit, Santosh
Noa, Francoise M. Amombo
Zhang, Jian
Gao, Wengeng
Rahimi, Shadi
Öhrström, Lars
Mijakovic, Ivan
Biological Physics
Mechano-bactericidal (MB) surfaces have been proposed as an emerging strategy for preventing biofilm formation. Unlike antibiotics and metal ions that chemically interfere with cellular processes, MB nanostructures cause physical damage to the bacteria. The antibacterial performance of artificial MB surfaces relies on rational control of surface features, which is difficult to achieve for large surfaces in real-life applications. Herein, we report a facile and scalable method for fabricating MB surfaces based on metal-organic frameworks (MOFs) using epitaxial MOF-on-MOF hybrids as building blocks with nanopillars of less than 5 nm tip diameter, 200 nm base diameter, and 300 nm length. Two methods of MOF surface assembly, in-situ growth and ex-situ dropcasting, result in surfaces with nanopillars in different orientations, both presenting MB actions (bactericidal efficiency of 83% for E. coli). Distinct MB mechanisms, including stretching, impaling, and apoptosis-like death induced by mechanical injury are discussed with the observed bacterial morphology on the obtained MOF surfaces.
title Mechano-Bactericidal Surfaces Achieved by Epitaxial Growth of Metal-Organic Frameworks
topic Biological Physics
url https://arxiv.org/abs/2503.16003