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Main Authors: Buglakova, Elena, Archit, Anwai, D'Imprima, Edoardo, Mahamid, Julia, Pape, Constantin, Kreshuk, Anna
Format: Preprint
Published: 2025
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Online Access:https://arxiv.org/abs/2503.19545
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author Buglakova, Elena
Archit, Anwai
D'Imprima, Edoardo
Mahamid, Julia
Pape, Constantin
Kreshuk, Anna
author_facet Buglakova, Elena
Archit, Anwai
D'Imprima, Edoardo
Mahamid, Julia
Pape, Constantin
Kreshuk, Anna
contents Segmentation of very large images is a common problem in microscopy, medical imaging or remote sensing. The problem is usually addressed by sliding window inference, which can theoretically lead to seamlessly stitched predictions. However, in practice many of the popular pipelines still suffer from tiling artifacts. We investigate the root cause of these issues and show that they stem from the normalization layers within the neural networks. We propose indicators to detect normalization issues and further explore the trade-offs between artifact-free and high-quality predictions, using three diverse microscopy datasets as examples. Finally, we propose to use BatchRenorm as the most suitable normalization strategy, which effectively removes tiling artifacts and enhances transfer performance, thereby improving the reusability of trained networks for new datasets.
format Preprint
id arxiv_https___arxiv_org_abs_2503_19545
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Tiling artifacts and trade-offs of feature normalization in the segmentation of large biological images
Buglakova, Elena
Archit, Anwai
D'Imprima, Edoardo
Mahamid, Julia
Pape, Constantin
Kreshuk, Anna
Computer Vision and Pattern Recognition
Segmentation of very large images is a common problem in microscopy, medical imaging or remote sensing. The problem is usually addressed by sliding window inference, which can theoretically lead to seamlessly stitched predictions. However, in practice many of the popular pipelines still suffer from tiling artifacts. We investigate the root cause of these issues and show that they stem from the normalization layers within the neural networks. We propose indicators to detect normalization issues and further explore the trade-offs between artifact-free and high-quality predictions, using three diverse microscopy datasets as examples. Finally, we propose to use BatchRenorm as the most suitable normalization strategy, which effectively removes tiling artifacts and enhances transfer performance, thereby improving the reusability of trained networks for new datasets.
title Tiling artifacts and trade-offs of feature normalization in the segmentation of large biological images
topic Computer Vision and Pattern Recognition
url https://arxiv.org/abs/2503.19545