Fungicides vs mycoinsecticides in the management of corn leafhopper: physicochemical, in vitro and in vivo compatibilities, and degradation kinetics in maize plants
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| Natura: | Preprint |
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2025
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| _version_ | 1866909555309412352 |
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| author | Rakes, Matheus Morais, Maíra Chagas Sperotto, Maria Eduarda Zanardi, Odimar Zanuzo Palma, Gabriel Rodrigues Floriano, Luana Zanella, Renato Prestes, Osmar Damian Bernardi, Daniel Grützmacher, Anderson Dionei Ribeiro, Leandro do Prado |
| author_facet | Rakes, Matheus Morais, Maíra Chagas Sperotto, Maria Eduarda Zanardi, Odimar Zanuzo Palma, Gabriel Rodrigues Floriano, Luana Zanella, Renato Prestes, Osmar Damian Bernardi, Daniel Grützmacher, Anderson Dionei Ribeiro, Leandro do Prado |
| contents | The present study investigates the compatibility of mycoinsecticides based on isolates IBCB66 and Simbi BB15 of Beauveria bassiana and Esalq-1296 of Cordyceps javanica, which are registered for the management of Dalbulus maidis in Brazil, with synthetic fungicides. Irrespective of the fungicide, a total inhibition in the number of colony-forming units (CFUs), vegetative growth, conidiogenesis, and conidial viability of the three tested isolates was observed, with their incompatibility being indicated in the in vitro bioassays. However, the use of formulated mycoinsecticides mitigated the impact of these xenobiotics on the number of CFUs, with the commercial mycoinsecticide FlyControl (B. bassiana isolate Simbi BB15) being the least sensitive to the fungicides propiconazole + difenoconazole, bixafem + prothioconazole + trifloxystrobin and trifloxystrobin + tebuconazole. Nevertheless, an increase in exposure time (from 1.5 to 3 hours) generally led to an increase in the toxicity of fungicides towards entomopathogens. Physical-chemical compatibility assessments indicated that physical incompatibilities were observed, depending on the mycoinsecticide formulation. In addition, in vivo bioassays employing D. maidis adults demonstrated that, despite a synergistic effect on mortality in certain binary mixtures, no cadavers exposed to such mixtures exhibited fungal extrusion. Furthermore, analyses using UHPLC/MS/MS revealed alterations in the degradation kinetics (k) of the active ingredient (a.i.) pyraclostrobin, with changes greater than tenfold being observed in the different formulations of the fungicides that were tested. Consequently, given the diminished degradation kinetics of the active ingredients in maize plants, the implementation of mycoinsecticides should precede, in isolation, the application of synthetic fungicides within the framework of phytosanitary management of maize crops. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2503_21606 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Fungicides vs mycoinsecticides in the management of corn leafhopper: physicochemical, in vitro and in vivo compatibilities, and degradation kinetics in maize plants Rakes, Matheus Morais, Maíra Chagas Sperotto, Maria Eduarda Zanardi, Odimar Zanuzo Palma, Gabriel Rodrigues Floriano, Luana Zanella, Renato Prestes, Osmar Damian Bernardi, Daniel Grützmacher, Anderson Dionei Ribeiro, Leandro do Prado Quantitative Methods The present study investigates the compatibility of mycoinsecticides based on isolates IBCB66 and Simbi BB15 of Beauveria bassiana and Esalq-1296 of Cordyceps javanica, which are registered for the management of Dalbulus maidis in Brazil, with synthetic fungicides. Irrespective of the fungicide, a total inhibition in the number of colony-forming units (CFUs), vegetative growth, conidiogenesis, and conidial viability of the three tested isolates was observed, with their incompatibility being indicated in the in vitro bioassays. However, the use of formulated mycoinsecticides mitigated the impact of these xenobiotics on the number of CFUs, with the commercial mycoinsecticide FlyControl (B. bassiana isolate Simbi BB15) being the least sensitive to the fungicides propiconazole + difenoconazole, bixafem + prothioconazole + trifloxystrobin and trifloxystrobin + tebuconazole. Nevertheless, an increase in exposure time (from 1.5 to 3 hours) generally led to an increase in the toxicity of fungicides towards entomopathogens. Physical-chemical compatibility assessments indicated that physical incompatibilities were observed, depending on the mycoinsecticide formulation. In addition, in vivo bioassays employing D. maidis adults demonstrated that, despite a synergistic effect on mortality in certain binary mixtures, no cadavers exposed to such mixtures exhibited fungal extrusion. Furthermore, analyses using UHPLC/MS/MS revealed alterations in the degradation kinetics (k) of the active ingredient (a.i.) pyraclostrobin, with changes greater than tenfold being observed in the different formulations of the fungicides that were tested. Consequently, given the diminished degradation kinetics of the active ingredients in maize plants, the implementation of mycoinsecticides should precede, in isolation, the application of synthetic fungicides within the framework of phytosanitary management of maize crops. |
| title | Fungicides vs mycoinsecticides in the management of corn leafhopper: physicochemical, in vitro and in vivo compatibilities, and degradation kinetics in maize plants |
| topic | Quantitative Methods |
| url | https://arxiv.org/abs/2503.21606 |