Anisotropy analysis of bamboo and tooth using 4-angle polarization micro-spectroscopy
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| Main Authors: | , , , , , , , , |
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| Format: | Preprint |
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2025
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| author | Ryu, Meguya Huang, Hsin-Hui Vongsvivut, Jitraporn Ng, Soon Hock Dumbryte, Irma Narbutis, Donatas Malinauskas, Mangirdas Juodkazis, Saulius Morikawa, Junko |
| author_facet | Ryu, Meguya Huang, Hsin-Hui Vongsvivut, Jitraporn Ng, Soon Hock Dumbryte, Irma Narbutis, Donatas Malinauskas, Mangirdas Juodkazis, Saulius Morikawa, Junko |
| contents | To investigate the anisotropic properties of biomaterials, two distinct classes are considered: polymer-based (e.g., cellulose in plants) and crystalline-based (e.g., enamel in teeth), each demonstrating distinct structural and functional characteristics. Four-angle polarization (4-pol.) spectral mapping of sub-1 μm bamboo slices was carried out in the mid-IR spectral range (2.5-20 μm) to reveal the 3D organization of the chemical bonding of cellulose using the key characteristic absorption bands associated with C-O-C and C-N vibrational modes. The longitudinal and transverse microtome slices revealed a switch between the presence and absence of dichroism in parenchyma cell walls and vascular bundles. The cell wall showed continuous alignment of the C-O-C stretching vibrational mode (8.6 μm/1163 cm-1) down to the pixel resolution of ~ 4 μm (the step size in imaging) in the transverse slice; the cell wall thickness is ~ 1 μm. Thin microtomed slices of a tooth were measured in transmission and reflection modes. The single-point reflection measurements, performed using two perpendicular orientations, revealed orientational anisotropy in the enamel, which was absent in the dentin region. High sub-diffraction limited lateral resolution was numerically validated using a simplified-model of a Gaussian beam reading out material pixels with a defined orientation of absorption. It is shown that the orientation of small ~ λ/10 ~ 1 μm objects can be revealed using a focal spot of ~ λ/NA ~ 20 μm, defining the diffraction limit for the objective lens with a numerical aperture NA ~ 0.5. |
| format | Preprint |
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arxiv_https___arxiv_org_abs_2504_02711 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Anisotropy analysis of bamboo and tooth using 4-angle polarization micro-spectroscopy Ryu, Meguya Huang, Hsin-Hui Vongsvivut, Jitraporn Ng, Soon Hock Dumbryte, Irma Narbutis, Donatas Malinauskas, Mangirdas Juodkazis, Saulius Morikawa, Junko Biological Physics Medical Physics To investigate the anisotropic properties of biomaterials, two distinct classes are considered: polymer-based (e.g., cellulose in plants) and crystalline-based (e.g., enamel in teeth), each demonstrating distinct structural and functional characteristics. Four-angle polarization (4-pol.) spectral mapping of sub-1 μm bamboo slices was carried out in the mid-IR spectral range (2.5-20 μm) to reveal the 3D organization of the chemical bonding of cellulose using the key characteristic absorption bands associated with C-O-C and C-N vibrational modes. The longitudinal and transverse microtome slices revealed a switch between the presence and absence of dichroism in parenchyma cell walls and vascular bundles. The cell wall showed continuous alignment of the C-O-C stretching vibrational mode (8.6 μm/1163 cm-1) down to the pixel resolution of ~ 4 μm (the step size in imaging) in the transverse slice; the cell wall thickness is ~ 1 μm. Thin microtomed slices of a tooth were measured in transmission and reflection modes. The single-point reflection measurements, performed using two perpendicular orientations, revealed orientational anisotropy in the enamel, which was absent in the dentin region. High sub-diffraction limited lateral resolution was numerically validated using a simplified-model of a Gaussian beam reading out material pixels with a defined orientation of absorption. It is shown that the orientation of small ~ λ/10 ~ 1 μm objects can be revealed using a focal spot of ~ λ/NA ~ 20 μm, defining the diffraction limit for the objective lens with a numerical aperture NA ~ 0.5. |
| title | Anisotropy analysis of bamboo and tooth using 4-angle polarization micro-spectroscopy |
| topic | Biological Physics Medical Physics |
| url | https://arxiv.org/abs/2504.02711 |