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| Main Authors: | , , , , , , |
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| Format: | Preprint |
| Published: |
2025
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| Subjects: | |
| Online Access: | https://arxiv.org/abs/2504.04611 |
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| _version_ | 1866909729172750336 |
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| author | Duan, Yuqin Gu, Qiushi Wang, Hanfeng Hu, Yong Chen, Kevin C. Trusheim, Matthew E. Englund, Dirk R. |
| author_facet | Duan, Yuqin Gu, Qiushi Wang, Hanfeng Hu, Yong Chen, Kevin C. Trusheim, Matthew E. Englund, Dirk R. |
| contents | Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed localization below nanometer precision, by applying prior knowledge of correlated fluorescence emission from single emitters. However, achieving a refinement from 1 nm to 1 Angstrom demands a hundred-fold increase in collected photon signal. This quadratic resource scaling imposes a fundamental barrier in SMLM, where the intense photon collection is challenged by photo-bleaching, prolonged integration times, and inherent practical constraints. Here, we break this limit by harnessing the periodic nature of the atomic lattice structure. Applying this discrete grid imaging technique (DIGIT) in a quantum emitter system, we observe an exponential collapse of localization uncertainty once surpassing the host crystal's atomic lattice constant. We further applied DIGIT to a large-scale quantum emitter array, enabling parallel positioning of each emitter through wide-field imaging. Collectively, these advancements establish DIGIT as a competitive tool for achieving unprecedented, precise measurements, ultimately paving the way to direct optical resolution of crystal and atomic features within quantum and biological systems. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2504_04611 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | A Bayesian Approach towards Atomically-precise Localization in Fluorescence Microscopy Duan, Yuqin Gu, Qiushi Wang, Hanfeng Hu, Yong Chen, Kevin C. Trusheim, Matthew E. Englund, Dirk R. Optics Super-resolution microscopy has revolutionized the imaging of complex physical and biological systems by surpassing the Abbe diffraction limit. Recent advancements, particularly in single-molecule localization microscopy (SMLM), have pushed localization below nanometer precision, by applying prior knowledge of correlated fluorescence emission from single emitters. However, achieving a refinement from 1 nm to 1 Angstrom demands a hundred-fold increase in collected photon signal. This quadratic resource scaling imposes a fundamental barrier in SMLM, where the intense photon collection is challenged by photo-bleaching, prolonged integration times, and inherent practical constraints. Here, we break this limit by harnessing the periodic nature of the atomic lattice structure. Applying this discrete grid imaging technique (DIGIT) in a quantum emitter system, we observe an exponential collapse of localization uncertainty once surpassing the host crystal's atomic lattice constant. We further applied DIGIT to a large-scale quantum emitter array, enabling parallel positioning of each emitter through wide-field imaging. Collectively, these advancements establish DIGIT as a competitive tool for achieving unprecedented, precise measurements, ultimately paving the way to direct optical resolution of crystal and atomic features within quantum and biological systems. |
| title | A Bayesian Approach towards Atomically-precise Localization in Fluorescence Microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2504.04611 |