A Few-Shot Metric Learning Method with Dual-Channel Attention for Cross-Modal Same-Neuron Identification

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Autori principali: Li, Wenwei, Cai, Liyi, Chen, Wu, Li, Anan
Natura: Preprint
Pubblicazione: 2025
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author Li, Wenwei
Cai, Liyi
Chen, Wu
Li, Anan
author_facet Li, Wenwei
Cai, Liyi
Chen, Wu
Li, Anan
contents In neuroscience research, achieving single-neuron matching across different imaging modalities is critical for understanding the relationship between neuronal structure and function. However, modality gaps and limited annotations present significant challenges. We propose a few-shot metric learning method with a dual-channel attention mechanism and a pretrained vision transformer to enable robust cross-modal neuron identification. The local and global channels extract soma morphology and fiber context, respectively, and a gating mechanism fuses their outputs. To enhance the model's fine-grained discrimination capability, we introduce a hard sample mining strategy based on the MultiSimilarityMiner algorithm, along with the Circle Loss function. Experiments on two-photon and fMOST datasets demonstrate superior Top-K accuracy and recall compared to existing methods. Ablation studies and t-SNE visualizations validate the effectiveness of each module. The method also achieves a favorable trade-off between accuracy and training efficiency under different fine-tuning strategies. These results suggest that the proposed approach offers a promising technical solution for accurate single-cell level matching and multimodal neuroimaging integration.
format Preprint
id arxiv_https___arxiv_org_abs_2504_16520
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle A Few-Shot Metric Learning Method with Dual-Channel Attention for Cross-Modal Same-Neuron Identification
Li, Wenwei
Cai, Liyi
Chen, Wu
Li, Anan
Computer Vision and Pattern Recognition
Neurons and Cognition
In neuroscience research, achieving single-neuron matching across different imaging modalities is critical for understanding the relationship between neuronal structure and function. However, modality gaps and limited annotations present significant challenges. We propose a few-shot metric learning method with a dual-channel attention mechanism and a pretrained vision transformer to enable robust cross-modal neuron identification. The local and global channels extract soma morphology and fiber context, respectively, and a gating mechanism fuses their outputs. To enhance the model's fine-grained discrimination capability, we introduce a hard sample mining strategy based on the MultiSimilarityMiner algorithm, along with the Circle Loss function. Experiments on two-photon and fMOST datasets demonstrate superior Top-K accuracy and recall compared to existing methods. Ablation studies and t-SNE visualizations validate the effectiveness of each module. The method also achieves a favorable trade-off between accuracy and training efficiency under different fine-tuning strategies. These results suggest that the proposed approach offers a promising technical solution for accurate single-cell level matching and multimodal neuroimaging integration.
title A Few-Shot Metric Learning Method with Dual-Channel Attention for Cross-Modal Same-Neuron Identification
topic Computer Vision and Pattern Recognition
Neurons and Cognition
url https://arxiv.org/abs/2504.16520