Fifth Harmonic and Five-Photon Excitation Fluorescence Multiphoton Microscopy
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arXiv
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| Main Authors: | , , , , , |
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| Format: | Preprint |
| Published: |
2025
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| _version_ | 1866916812003737600 |
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| author | Magnus, Joshua H. Nguyen, Lam T. Alevy, Elana G. Sawyer, Travis W. Crossley, Samuel D. Kieu, Khanh |
| author_facet | Magnus, Joshua H. Nguyen, Lam T. Alevy, Elana G. Sawyer, Travis W. Crossley, Samuel D. Kieu, Khanh |
| contents | We report the application of fifth order nonlinear optical processes for multiphoton microscopy, including fifth harmonic and 5-photon excitation fluorescence. This novel imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth harmonic and 5-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth, while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2504_20309 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Fifth Harmonic and Five-Photon Excitation Fluorescence Multiphoton Microscopy Magnus, Joshua H. Nguyen, Lam T. Alevy, Elana G. Sawyer, Travis W. Crossley, Samuel D. Kieu, Khanh Optics We report the application of fifth order nonlinear optical processes for multiphoton microscopy, including fifth harmonic and 5-photon excitation fluorescence. This novel imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth harmonic and 5-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth, while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing. |
| title | Fifth Harmonic and Five-Photon Excitation Fluorescence Multiphoton Microscopy |
| topic | Optics |
| url | https://arxiv.org/abs/2504.20309 |