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Bibliographic Details
Main Authors: Magnus, Joshua H., Nguyen, Lam T., Alevy, Elana G., Sawyer, Travis W., Crossley, Samuel D., Kieu, Khanh
Format: Preprint
Published: 2025
Subjects:
Online Access:https://arxiv.org/abs/2504.20309
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Table of Contents:
  • We report the application of fifth order nonlinear optical processes for multiphoton microscopy, including fifth harmonic and 5-photon excitation fluorescence. This novel imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth harmonic and 5-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth, while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing.