Pupil Phase Series: A Fast, Accurate, and Energy-Conserving Model for Forward and Inverse Light Scattering in Thick Biological Samples
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arXiv
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| Main Authors: | , , , |
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| Format: | Preprint |
| Published: |
2025
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| _version_ | 1866910922764713984 |
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| author | Hugonnet, Herve Oh, Chulmin Park, Juyeon Park, YongKeun |
| author_facet | Hugonnet, Herve Oh, Chulmin Park, Juyeon Park, YongKeun |
| contents | We present the pupil phase series (PPS), a fast and accurate forward scattering algorithm for simulating and inverting multiple light scattering in large biological samples. PPS achieves high-angle scattering accuracy and energy conservation simultaneously by introducing a spatially varying phase modulation in the pupil plane. By expanding the scattering term into a Taylor series, PPS achieves high precision while maintaining computational efficiency. We integrate PPS into a quasi-Newton inverse solver to reconstruct the three-dimensional refractive index of a 180 um-thick human organoid. Compared to linear reconstruction, our method recovers subcellular features-such as nuclei and vesicular structures-deep within the sample volume. PPS offers a scalable and interpretable alternative to conventional solvers, paving the way for high-throughput, label-free imaging of optically thick biological tissues. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2504_21369 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Pupil Phase Series: A Fast, Accurate, and Energy-Conserving Model for Forward and Inverse Light Scattering in Thick Biological Samples Hugonnet, Herve Oh, Chulmin Park, Juyeon Park, YongKeun Optics We present the pupil phase series (PPS), a fast and accurate forward scattering algorithm for simulating and inverting multiple light scattering in large biological samples. PPS achieves high-angle scattering accuracy and energy conservation simultaneously by introducing a spatially varying phase modulation in the pupil plane. By expanding the scattering term into a Taylor series, PPS achieves high precision while maintaining computational efficiency. We integrate PPS into a quasi-Newton inverse solver to reconstruct the three-dimensional refractive index of a 180 um-thick human organoid. Compared to linear reconstruction, our method recovers subcellular features-such as nuclei and vesicular structures-deep within the sample volume. PPS offers a scalable and interpretable alternative to conventional solvers, paving the way for high-throughput, label-free imaging of optically thick biological tissues. |
| title | Pupil Phase Series: A Fast, Accurate, and Energy-Conserving Model for Forward and Inverse Light Scattering in Thick Biological Samples |
| topic | Optics |
| url | https://arxiv.org/abs/2504.21369 |