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| Format: | Preprint |
| Veröffentlicht: |
2025
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| Online-Zugang: | https://arxiv.org/abs/2504.21434 |
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| _version_ | 1866908343884316672 |
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| author | Helmi, Seham Benson, Erik Thiele, Jan Christoph Moulin, Emilie Giuseppone, Nicolas Turberfield, Andrew J |
| author_facet | Helmi, Seham Benson, Erik Thiele, Jan Christoph Moulin, Emilie Giuseppone, Nicolas Turberfield, Andrew J |
| contents | Synthetic molecular motors are an appealing means to control motion at the nanoscale, but understanding their behaviour as single-molecule actuators and integrating them into larger, functional systems remain technical challenges. Translating molecular actuation into coordinated device-level behaviour requires precise placement and orientation of the motors: DNA origami provides a powerful platform for positioning molecules with nanometre precision. Here, we demonstrate integration of a light-driven, rotary molecular motor into a DNA-based nanoscale actuator through site-specific, four-point conjugation. The motor is labelled with four distinct oligonucleotides, two on each side, using DNA-templated chemistry. This modular approach enables stable, oriented incorporation of the motor into a DNA assembly through DNA hybridization. Upon photoactivation with UV light, the motor transduces photon energy into rotary motion. By coupling the motor to a fluorescently labelled DNA rotor arm we amplify its movement and enable real-time observation using total internal reflection fluorescence microscopy. A subset of assembled devices exhibits light-induced conformational transitions and directional motion consistent with the expected photochemical mechanism. These results establish a programmable framework for integration of light-driven molecular motors into synthetic nanomachines and tools for the study of their behaviour. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2504_21434 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Integration of a Synthetic Molecular Motor Into a Rotary DNA Nanostructure: A Framework for Single-Molecule Actuation Helmi, Seham Benson, Erik Thiele, Jan Christoph Moulin, Emilie Giuseppone, Nicolas Turberfield, Andrew J Applied Physics Synthetic molecular motors are an appealing means to control motion at the nanoscale, but understanding their behaviour as single-molecule actuators and integrating them into larger, functional systems remain technical challenges. Translating molecular actuation into coordinated device-level behaviour requires precise placement and orientation of the motors: DNA origami provides a powerful platform for positioning molecules with nanometre precision. Here, we demonstrate integration of a light-driven, rotary molecular motor into a DNA-based nanoscale actuator through site-specific, four-point conjugation. The motor is labelled with four distinct oligonucleotides, two on each side, using DNA-templated chemistry. This modular approach enables stable, oriented incorporation of the motor into a DNA assembly through DNA hybridization. Upon photoactivation with UV light, the motor transduces photon energy into rotary motion. By coupling the motor to a fluorescently labelled DNA rotor arm we amplify its movement and enable real-time observation using total internal reflection fluorescence microscopy. A subset of assembled devices exhibits light-induced conformational transitions and directional motion consistent with the expected photochemical mechanism. These results establish a programmable framework for integration of light-driven molecular motors into synthetic nanomachines and tools for the study of their behaviour. |
| title | Integration of a Synthetic Molecular Motor Into a Rotary DNA Nanostructure: A Framework for Single-Molecule Actuation |
| topic | Applied Physics |
| url | https://arxiv.org/abs/2504.21434 |