Efficiently driving F$_1$ molecular motor in experiment by suppressing nonequilibrium variation
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arXiv
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| Auteurs principaux: | , , , , , , |
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| Format: | Preprint |
| Publié: |
2025
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| _version_ | 1866916995616735232 |
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| author | Mishima, Takahide Gupta, Deepak Nakayama, Yohei Wareham, W. Callum Ohyama, Takumi Sivak, David A. Toyabe, Shoichi |
| author_facet | Mishima, Takahide Gupta, Deepak Nakayama, Yohei Wareham, W. Callum Ohyama, Takumi Sivak, David A. Toyabe, Shoichi |
| contents | F$_1$-ATPase (F$_1$) is central to cellular energy transduction. Forcibly rotated by another motor F$_\mathrm{o}$, F$_1$ catalyzes ATP synthesis by converting mechanical work into chemical free energy stored in the molecule ATP. The details of how F$_\mathrm{o}$ drives F$_1$ are not fully understood; however, evaluating efficient ways to rotate F$_1$ could provide fruitful insights into this driving since there is a selective pressure to improve efficiency. Here, we show that rotating F$_1$ with an angle clamp is significantly more efficient than a constant torque. Our experiments, combined with theory and simulation, indicate that the angle clamp significantly suppresses the nonequilibrium variation that contributes to the futile dissipation of input work. |
| format | Preprint |
| id |
arxiv_https___arxiv_org_abs_2505_01101 |
| institution | arXiv |
| publishDate | 2025 |
| record_format | arxiv |
| spellingShingle | Efficiently driving F$_1$ molecular motor in experiment by suppressing nonequilibrium variation Mishima, Takahide Gupta, Deepak Nakayama, Yohei Wareham, W. Callum Ohyama, Takumi Sivak, David A. Toyabe, Shoichi Biological Physics Statistical Mechanics F$_1$-ATPase (F$_1$) is central to cellular energy transduction. Forcibly rotated by another motor F$_\mathrm{o}$, F$_1$ catalyzes ATP synthesis by converting mechanical work into chemical free energy stored in the molecule ATP. The details of how F$_\mathrm{o}$ drives F$_1$ are not fully understood; however, evaluating efficient ways to rotate F$_1$ could provide fruitful insights into this driving since there is a selective pressure to improve efficiency. Here, we show that rotating F$_1$ with an angle clamp is significantly more efficient than a constant torque. Our experiments, combined with theory and simulation, indicate that the angle clamp significantly suppresses the nonequilibrium variation that contributes to the futile dissipation of input work. |
| title | Efficiently driving F$_1$ molecular motor in experiment by suppressing nonequilibrium variation |
| topic | Biological Physics Statistical Mechanics |
| url | https://arxiv.org/abs/2505.01101 |