Topology across Scales on Heterogeneous Cell Data

Fuente: arXiv
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Main Authors: Torras-Pérez, Maria, Yoon, Iris H. R., Weeratunga, Praveen, Ho, Ling-Pei, Byrne, Helen M., Tillmann, Ulrike, Harrington, Heather A.
Format: Preprint
Published: 2025
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author Torras-Pérez, Maria
Yoon, Iris H. R.
Weeratunga, Praveen
Ho, Ling-Pei
Byrne, Helen M.
Tillmann, Ulrike
Harrington, Heather A.
author_facet Torras-Pérez, Maria
Yoon, Iris H. R.
Weeratunga, Praveen
Ho, Ling-Pei
Byrne, Helen M.
Tillmann, Ulrike
Harrington, Heather A.
contents Multiplexed imaging allows multiple cell types to be simultaneously visualised in a single tissue sample, generating unprecedented amounts of spatially-resolved, biological data. In topological data analysis, persistent homology provides multiscale descriptors of ``shape" suitable for the analysis of such spatial data. Here we propose a novel visualisation of persistence homology (PH) and fine-tune vectorisations thereof (exploring the effect of different weightings for persistence images, a prominent vectorisation of PH). These approaches offer new biological interpretations and promising avenues for improving the analysis of complex spatial biological data especially in multiple cell type data. To illustrate our methods, we apply them to a lung data set from fatal cases of COVID-19 and a data set from lupus murine spleen.
format Preprint
id arxiv_https___arxiv_org_abs_2505_02717
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle Topology across Scales on Heterogeneous Cell Data
Torras-Pérez, Maria
Yoon, Iris H. R.
Weeratunga, Praveen
Ho, Ling-Pei
Byrne, Helen M.
Tillmann, Ulrike
Harrington, Heather A.
Quantitative Methods
Algebraic Topology
55N31, 62R40, 92-08
Multiplexed imaging allows multiple cell types to be simultaneously visualised in a single tissue sample, generating unprecedented amounts of spatially-resolved, biological data. In topological data analysis, persistent homology provides multiscale descriptors of ``shape" suitable for the analysis of such spatial data. Here we propose a novel visualisation of persistence homology (PH) and fine-tune vectorisations thereof (exploring the effect of different weightings for persistence images, a prominent vectorisation of PH). These approaches offer new biological interpretations and promising avenues for improving the analysis of complex spatial biological data especially in multiple cell type data. To illustrate our methods, we apply them to a lung data set from fatal cases of COVID-19 and a data set from lupus murine spleen.
title Topology across Scales on Heterogeneous Cell Data
topic Quantitative Methods
Algebraic Topology
55N31, 62R40, 92-08
url https://arxiv.org/abs/2505.02717