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Main Authors: Zhang, Zhonghai, Li, Yewen, Meng, Ke, Zhang, Chunming, Tan, Guangming
Format: Preprint
Published: 2025
Subjects:
Online Access:https://arxiv.org/abs/2505.06127
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author Zhang, Zhonghai
Li, Yewen
Meng, Ke
Zhang, Chunming
Tan, Guangming
author_facet Zhang, Zhonghai
Li, Yewen
Meng, Ke
Zhang, Chunming
Tan, Guangming
contents Duplicate marking is a critical preprocessing step in gene sequence analysis to flag redundant reads arising from polymerase chain reaction(PCR) amplification and sequencing artifacts. Although Picard MarkDuplicates is widely recognized as the gold-standard tool, its single-threaded implementation and reliance on global sorting result in significant computational and resource overhead, limiting its efficiency on large-scale datasets. Here, we introduce FastDup: a high-performance, scalable solution that follows the speculation-and-test mechanism. FastDup achieves up to 20x throughput speedup and guarantees 100\% identical output compared to Picard MarkDuplicates. FastDup is a C++ program available from GitHub (https://github.com/zzhofict/FastDup.git) under the MIT license.
format Preprint
id arxiv_https___arxiv_org_abs_2505_06127
institution arXiv
publishDate 2025
record_format arxiv
spellingShingle FastDup: a scalable duplicate marking tool using speculation-and-test mechanism
Zhang, Zhonghai
Li, Yewen
Meng, Ke
Zhang, Chunming
Tan, Guangming
Genomics
Duplicate marking is a critical preprocessing step in gene sequence analysis to flag redundant reads arising from polymerase chain reaction(PCR) amplification and sequencing artifacts. Although Picard MarkDuplicates is widely recognized as the gold-standard tool, its single-threaded implementation and reliance on global sorting result in significant computational and resource overhead, limiting its efficiency on large-scale datasets. Here, we introduce FastDup: a high-performance, scalable solution that follows the speculation-and-test mechanism. FastDup achieves up to 20x throughput speedup and guarantees 100\% identical output compared to Picard MarkDuplicates. FastDup is a C++ program available from GitHub (https://github.com/zzhofict/FastDup.git) under the MIT license.
title FastDup: a scalable duplicate marking tool using speculation-and-test mechanism
topic Genomics
url https://arxiv.org/abs/2505.06127